12.15 Effect of Anti-Hypertensive Treatment on Circulating Endothelial Progenitor Cells in Patients with Mild Essential
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High Blood Press Cardiovasc Prev 2008; 15 (3): 171-215 1120-9879/08/0003-0171/$48.00/0 © 2008 Adis Data Information BV. All rights reserved.
Vessels and Endothelium 12.15 Effect of Anti-Hypertensive Treatment on Circulating Endothelial Progenitor Cells in Patients with Mild Essential Hypertension C. De Ciuceis (1), E. Porteri (1), D. Rizzoni (1), M.L. Muiesan (1), M. Salvetti (1), A. Paini (1), E. Belotti (1), F. Zani (1), G.E.M. Boari (1), E. Agabiti Rosei (1) ` degli Studi di (1)Dipartimento di Scienze Mediche e Chirurgiche, Universita Brescia, Brescia, Italy Introduction. It has been reported that circulating endothelial progenitor cells (EPC) number reflects the endogenous vascular repair ability with the EPCs pool declining in presence of cardiovascular risk factors. However, their relationship with hypertension and the effect of anti-hypertensive treatment remains unclear. Methods. Fifteen patients with mild essential hypertension were randomized to receive barnidipine (BD dose up to 20 mg) or hydrochlorothiazide (HCT dose up to 25 mg). Circulating EPC were isolated from peripheral blood at baseline and after 3 months of treatment. Mononuclear cells (MNC) were cultured with endothelial basal medium supplemented with EGM Single Quots. Fluorescent chemical detection of EPCs was performed on attached MNC after 7 days. EPC were identified by positive double staining for both FITC-labelled Ulex europaeus agglutinin I and Dil-labelled acetylated low density lipoprotein. EPC were counted with an inverted fluorescent microscope (cell number/ 10^7plated cells per 1.17 mm2 of area). Results. After 3 months of treatment systolic and diastolic blood pressure (BP) was reduced from 143.13±3.0 to 130.20±1.6 and from 94.93±1.8 to 82.60±2.0 mm Hg, respectively (p
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