A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved
- PDF / 1,100,328 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 1 Downloads / 216 Views
ORIGINAL ARTICLE
A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved mouse spermatogonial stem cells Afshin Pirnia1 · Vahideh Assadollahi2 · Masoud Alasvand2 · Arezou Moghadam1 · Mohammad Reza Gholami3 Received: 2 October 2020 / Accepted: 11 November 2020 © Springer Nature B.V. 2020
Abstract Serum is a common supplement that is widely used to protect various cells and tissues from cryopreservation because it provides the necessary active components for cell growth and maintenance. In this study, we compared the effects of newborn calf serum (NCS) and fetal bovine serum (FBS) on the cryopreservation of mouse spermatogonial stem cells (SSCs). The isolated SSCs were cryopreserved in two groups: freezing medium that contained 10% DMSO (dimethyl sulfoxide) and 10% FBS in DMEM (Dulbecco’s Modified Eagle’s Medium) (group 1) and freezing medium that contained 10% DMSO and 10% NCS in DMEM (group 2). Real-time PCR was performed for stemness gene expression. The SSCs’ viability was performed by trypan blue. We observed that the SSCs had increased viability in the NCS-freeze/thaw group (87.82%) compared to the FBS-freeze/thaw group (79.83%), but this increase was not statistically significant (P 3′)
Product length (bp)
Nanog
F: GGAGGACTTTCTGCAGCCTT R: ACCTGGTGGAGTCACAGAGT F: CTGTCAGGATTGCTCCCTGG R: AGGTAGCAAGGTGCTGTTCC F: CTGTCCAGGCATGGGAACAT R: GTGAGGTAGGTTTGTGGCGA F: CAATGTGTCCGTCGTGGATCT R: GTCCTCAGTGTAGCCCAAGATG F: ACATGGCTGTCTTCTGTCTG R: GGTGGTCACCTGTATGTGAG
154
Dazl Lin28 Gapdh Plzf
103 196 208 174
13
Molecular Biology Reports
Discussion
Fig. 2 Cellular viability frequency (%). Comparison of FBS-frozen/ thawed and NCS-frozen/thawed SSCs groups. The error bar is SEM. SEM standard error of means, ANOVA analysis of variance. Values are presented as mean ± SD (n = 3; P
Data Loading...
