Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-deri
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ORIGINAL ARTICLE
Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cordderived mesenchymal stem cells Maryam Samareh Salavati Pour . Reza Vahidi . Mahla Lashkari . Ali Derakhshani . Zahra Ameri . Alireza Farsinejad
Received: 12 October 2019 / Accepted: 31 May 2020 Ó Springer Nature B.V. 2020
Abstract As a widely used cell culture supplement, fetal bovine serum (FBS) harbor high content of growth, proliferation, and adhesion factors. However, high cost, bio-safety, possible xenogeneic agent transmission, finite accessible, and ethical controversy are major obstacles that discourage the use of this additive. Accordingly, novel alternatives have been proposed with various pros and cons. Still, caution should be taken in choosing suitable substitute given that the alteration in the main aspects of cultured cells
can be biased the consequences of clinical applications. Herein, the authors evaluated the impact of cord blood serum harvesting by hydroxyethyl starch (CBSHES), as an enriched source of growth factors, on the basic mesenchymal stem cells (MSCs) characteristics. In the present experiment, umbilical cord-derived MSCs were isolated and continuously nourished with Dulbecco’s Modified Eagle Medium containing either 10, 15, and 20% CBS-HES or FBSs to compare their morphology, immunophenotype, growth and
M. Samareh Salavati Pour Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran e-mail: [email protected]
M. Lashkari e-mail: [email protected]
R. Vahidi (&) A. Derakhshani Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran e-mail: [email protected]
Z. Ameri e-mail: [email protected]
M. Samareh Salavati Pour R. Vahidi M. Lashkari A. Derakhshani Z. Ameri A. Farsinejad (&) Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran e-mail: [email protected]
A. Derakhshani e-mail: [email protected]
M. Samareh Salavati Pour M. Lashkari Z. Ameri A. Farsinejad Department of Hematology and Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran
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Cytotechnology
proliferation rate, death rate, cell cycle, and gene expression profiles. Although all enriched media supported the expansion of MSCs with comparable morphology, cell surface markers, death rate, c-MYC and p16 expression, and growth rate, CBS-HES treated cells significantly (P \ 0.05) expressed more hTERT gene in a concentration-dependent manner. Yet no significant shift was observed in the cell cycle of cultured cells using the same concentrations of additives, a finding which further confirmed by Ki-67 immunostaining. CBS-HES as an available and affordable additive, seems to be an optimal, relatively safe, and promising FBS alternative for cultivation, propagation, and subsequent clinical applications of MSCs. Keywords
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