Bio-synthesis of silver nanoparticles with the brackish water blue-green alga Oscillatoria princeps and antibacterial as
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ORIGINAL ARTICLE
Bio‑synthesis of silver nanoparticles with the brackish water blue‑green alga Oscillatoria princeps and antibacterial assessment Ajit Kumar Bishoyi1 · Chita Ranjan Sahoo1 · Arpita Priyadarshinee Sahoo1 · Rabindra Nath Padhy1 Received: 13 July 2020 / Accepted: 17 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract This study describes the morphology of a filamentous non-heterocystous blue-green alga (cyanobacterium) Oscillatoria princeps isolated from the Bhabakundalswara sea mouth, its use in biogenic synthesis of silver nanoparticles (AgNPs), along with antimicrobial studies using multidrug resistant (MDR) methicillin-resistant strains of Staphylococcus aureus (MRSA), Streptococcus pyogenes and Escherichia coli, isolated from clinical samples. The synthesized O. princeps -AgNPs were characterized with UV–visible spectrometry, scanning electron microscopy (SEM), X-ray diffraction (XRD), dynamic light scattering analysis (DLS) and Fourier transform infrared spectroscopy (FT-IR) analyses. The synthesized AgNPs had remarkable antibacterial activities against MDR strains of MRSA, S. pyogenes and E. coli with the inhibitory zone sizes, 14–16 mm as recorded from agar well diffusion method, while MIC values were 100, 80, 60 µg/ml, for the respective cited bacterial species. Thus, O. princeps -AgNPs in future would be druggable antibacterials. Keywords Blue-green alga · Cyanobacterium · Oscillatoria sp. · Silver nanoparticles · Antibacterial activity
Introduction Two Gram-positive (GP) bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pyogenes, as well as, the Gram negative bacterium Escherichia coli are the notorious bacterial pathogens causing infections at several innards; even the former is known as the superbug in the health domain for its commonplace occurrence (Mishra et al. 2013). As a firmicute, S. aureus naturally occurs as a commensal in soft parts of body, e.g., skin, perineum, anterior nares of nose and pharynx, but it causes infection upon an entry to body (Dubey et al. 2013; McNeil and Fritz 2019). By the by, these two GP bacteria cause infections mostly in the upper respiratory tract (URT) (Kumpitsch et al. 2019; van den Broek et al. 2019). Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13204-020-01593-7) contains supplementary material, which is available to authorized users. * Rabindra Nath Padhy [email protected] 1
Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan (Deemed To Be University), Kalinga Nagar, Bhubaneswar, Odisha 751003, India
Furthermore, E. coli is a common bacterium causing infections in innards including urinary tract and URT (Jang et al. 2017; Adebisi et al. 2017); E. coli too causes toxic shock syndrome in immune-compromised patients (Tilahun et al. 2014). Since these aggressively infective pathogenic bacteria have become multidrug resistant (MDR) in the last several decades progressively, cause ghoulish ep
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