Clostridium difficile Methods and Protocols
This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and trans
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Adam P. Roberts Peter Mullany Editors
Clostridium difficile Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Clostridium difficile Methods and Protocols Second Edition
Edited by
Adam P. Roberts and Peter Mullany Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK
Editors Adam P. Roberts Department of Microbial Diseases UCL Eastman Dental Institute University College London London, UK
Peter Mullany Department of Microbial Diseases UCL Eastman Dental Institute University College London London, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6359-1 ISBN 978-1-4939-6361-4 (eBook) DOI 10.1007/978-1-4939-6361-4 Library of Congress Control Number: 2016941338 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface This is an update of the highly successful book first published in 2010 describing the available methods for the study of the important human pathogen Clostridium difficile. This book is required as the methods available to study the organism have moved forward at an impressive rate. Here, leading authors in the field describe methods used in their laboratories for investigating the physiology of the pathogen, which is vital if we are to thoroughly understand the diseases it causes. Excitingly, the experimental techniques used to investigate potential new treatments, including vaccine development, bacteriophage and phage product therapy, and fecal transplantation, are detailed as are the techniques used to analyze the various different mechanisms of horizontal gen
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