Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative
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Genes & Genomics https://doi.org/10.1007/s13258-020-00976-y
RESEARCH ARTICLE
Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach So‑Ra Han1 · Sung‑Min Jang2 · Young Min Chi2 · Byeollee Kim1 · Sang‑Hee Jung3 · Yung Mi Lee4 · Jun Uetake5 · Jun Hyuck Lee6,7 · Hyun Park2 · Tae‑Jin Oh1,8,9 Received: 20 January 2020 / Accepted: 17 July 2020 © The Genetics Society of Korea 2020
Abstract Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confirmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by finding new genes that degradation the pectin. Keywords Carbohydrate active enzyme · Comparative genomics · Genome sequencing · Pectin degradation · Sphingobium sp.
Introduction So-Ra Han, Sung-Min Jang these two authors contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13258-020-00976-y) contains supplementary material, which is available to authorized users. * Hyun Park [email protected] * Tae‑Jin Oh [email protected] Extended author information available on the last page of the article
Bacteria of genus Sphingobium, family Sphingomonadaceae, phylum Pro
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