Complete Genome Sequence of Zearalenone Degrading Bacteria Bacillus velezensis A2
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Complete Genome Sequence of Zearalenone Degrading Bacteria Bacillus velezensis A2 Mingyang Wang1 · Sheng Huang1 · Jia Chen1 · Si Chen1 · Miao Long1 Received: 31 January 2020 / Accepted: 28 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Zearalenone (ZEN) is a severe contaminant mycotoxin found worldwide. Our previous results indicate that Bacillus velezensis A2 isolated from ZEN-contaminated crop soil can degrade ZEN. Here, we present the complete genomic sequence of B. velezensis A2 (the Genbank accession number: CP053717), which contains 3,929,218 bp in the chromosome, has a GC content of 46.5%, and contains the encoded ZEN-degrading enzyme gene. The complete genomic sequence can provide a genomic basis for a series of A2 biotechnology applications as an effective method of degrading ZEN.
Introduction Zearalenone (ZEN) is a mycotoxin produced by Fusarium fungi, which damages cash crops [1], such as corn, wheat, and soybeans [2]. Since its chemical structure is similar to the reproductive toxicity of 17β-estradiol [3], when livestock consume feed containing ZEN, it will inflict reproductive damage such as abortion, infertility, and stillbirth of livestock [4]. Much research has been undertaken on the detoxification of ZEN in food and feed at home and abroad. Traditional ZEN detoxification methods often have some Mingyang Wang and Sheng Huang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02234-6) contains supplementary material, which is available to authorised users. * Miao Long [email protected] Mingyang Wang [email protected] Sheng Huang [email protected] Jia Chen [email protected] Si Chen [email protected] 1
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China
disadvantages. Compared with conventional detoxification methods, biological detoxification methods have the advantages of high specificity, high efficiency, and nontoxic metabolism. Numerous studies have shown that some bacteria can degrade ZEA [5]. These bacterial species include Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Saccharomyces cerevisiae [6]. Bacillus velezensis is a Gram-positive, sporeforming bacteria, and is usually found in soil and organic waste. Strains of this species are used as biodegradable bacteria because they can degrade some toxins and pollutants. Our previous studies have shown that B. velezensis A2 can detoxify ZEN [7]: B. velezensis A2 has been deposited with the China Centre for Type Culture Collection (CCTCC: M2018352); however, we did not analyse the detoxification process at gene-level. In this study, B. velezensis A2 was originally isolated from farmland soils contaminated with ZEN. Our previous results showed that the Gram-positive A2 strain is loc
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