Development of a One-Step Immunochromatographic Strip Test for Rapid Detection of Antibodies Against Classic Swine Fever
An immunochromatographic strip (GICA strip) was developed for the simple and rapid detection of antibodies against classical swine fever virus (CSFV). In the GICA strip, the expressed protein of E2 was labeled with colloidal gold and was used as the detec
- PDF / 304,135 Bytes
- 12 Pages / 439.37 x 666.142 pts Page_size
- 66 Downloads / 194 Views
Development of a One-Step Immunochromatographic Strip Test for Rapid Detection of Antibodies Against Classic Swine Fever Huiying Ren, Shun Zhou, Jianxin Wen, Xinmei Zhan, Wenhua Liu and Shangin Cui Abstract An immunochromatographic strip (GICA strip) was developed for the simple and rapid detection of antibodies against classical swine fever virus (CSFV). In the GICA strip, the expressed protein of E2 was labeled with colloidal gold and was used as the detector, and the staphylococcal protein A (SPA) and swine antibody against CSFV blotted on the nitrocellulose membrane were used for the test and control lines, respectively. Conjugation of E2 protein with colloidal gold was optimal at 5.25 lg of protein per mL of colloidal gold. The optimum concentration of the E2 protein applied at the test line was 1.5 mg/mL. The GICA strip was specific for antibodies against CSFV, produced negative results with sera from noninfected pigs or other animals, and was as sensitive or nearly as sensitive as ELISA and HI. According to the comparison of GICA strips with IDEXX ELISA kits, the coincidence rate was 93.26 %. The strips produced results within 5–15 min and can be stored at 4 C for 3 years or 37 C 1 year. The strip can be useful for both country veterinarians and field epidemiologists. Keywords Antibody
Classical swine fever Rapid test Strip
H. Ren (&) S. Zhou J. Wen X. Zhan W. Liu Qingdao Agricultural University, 266109 Qingdao, China e-mail: [email protected] S. Cui State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China e-mail: [email protected]
S. Li et al. (eds.), Frontier and Future Development of Information Technology in Medicine and Education, Lecture Notes in Electrical Engineering 269, DOI: 10.1007/978-94-007-7618-0_9, Springer Science+Business Media Dordrecht 2014
81
82
H. Ren et al.
9.1 Introduction The classical swine fever (CSF) caused by classical swine fever virus (CSFV), is a highly contagious viral infection of domestic pigs and wild boars, and also one of the most devastating porcine diseases worldwide (Moennig et al. 2003). The disease is endemic in Asia and common in many Central and South American countries as well as in Eastern Europe [5]. Though many countries (e.g., European Union member states) pursue a non-vaccination eradication policy, massive vaccination with attenuated vaccines, such as hog cholera lapinized vaccine (HCLV) (also known as C-strain), has been a major control strategy in China and many other developing countries [4]. In spite of these massive vaccinations, CSF outbreaks still occur in China, and remain as the main disease of swine in China. In recent years, non-typical or chronic CSF caused immunization failures have been frequently reported, mainly due to maternal antibody interference and immunotolerance caused by improper immunization [6]. Traditional methods for the diagnosis of CSFV infection and detection of antibodies
Data Loading...
