DNA Replication Methods and Protocols
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to sin
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Sonya Vengrova Jacob Dalgaard Editors
DNA Replication Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
DNA Replication Methods and Protocols Second Edition
Edited by
Sonya Vengrova Coventry, UK
Jacob Dalgaard Warwick Medical School, University of Warwick, Coventry, UK
Editors Sonya Vengrova Coventry, UK
Jacob Dalgaard Warwick Medical School University of Warwick Coventry, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2595-7 ISBN 978-1-4939-2596-4 (eBook) DOI 10.1007/978-1-4939-2596-4 Library of Congress Control Number: 2015935079 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The research field of DNA replication is progressing rapidly, and new technologies are developed every year, allowing researches to understand novel aspects of the complex processes of DNA replication. In this volume of “DNA Replication: Methods and Protocols” 17 method chapters are presented, which cover a range of systems, including mammalian, yeast, bacterial, and archaeal. The methods describe approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. This collection contains highly sited updated versions of methods first published in the previous edition as well as novel methods that have been developed in the recent years. The methods are described in a way that enables researchers new to the field to execute them; some of them can be performed using basic molecular biology equipment, while others require specialized se
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