Dual emission carbon dots as enzyme mimics and fluorescent probes for the determination of o -phenylenediamine and hydro
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ORIGINAL PAPER
Dual emission carbon dots as enzyme mimics and fluorescent probes for the determination of o-phenylenediamine and hydrogen peroxide Dhamodiran Mathivanan 1 & Sai Kumar Tammina 1 & Xiuli Wang 1 & Yaling Yang 1 Received: 28 September 2019 / Accepted: 31 March 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A selective and sensitive fluorescent technique is proposed to determine o-phenylenediamine (OPD) and hydrogen peroxide (H2O2). This is carried out by utilizing enzyme mimics carbon dots (N/Cl-CDs) and fluorescent probe carbon dots (N/Zn-CDs). The synthesized N/Cl-CDs and N/Zn-CDs were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and Ultravioletvisible spectrophotometry techniques. The particle size of synthesized carbon dots was found to be 4.1 ± 1.09 nm and 3.3 ± 1.82 nm for N/Cl-CDs and N/Zn-CDs, respectively. The N/Cl-CDs showed a noticeable intrinsic peroxidase-like activity, which could catalyze the oxidization of OPD by H2O2 to form the yellow colored product 2,3-diaminophenazine (DAP). The N/Zn-CD fluorescence was quenched directly by DAP through the inner filter effect (IFE). As a result, a novel double carbon dot system as enzyme mimics and fluorescent investigations to recognize OPD and H2O2 was obtained. The N/Cl-CDs were synthesized by deep eutectic solvent (DES) source and chamber electric furnace. N/Zn-CDs were also synthesized with the quantum yield of 27.52% through the tubular furnace technique using ethylenediamine tetra acetic acid disodium zinc salt and ascorbic acid as precursors. The detection limits were found to be 0.58 μM and 0.27 μM for OPD and H2O2, respectively. The nanoprobe was applied to real sample analysis, which showed excellent recovery in the range of 95.8–103.5% and 98.6–107.3% for OPD and H2O2, respectively. The dual emission carbon dots as enzyme mimic and fluorescent probe sensing system opens a new platform for determination of H2O2 and OPD in real samples. Keywords 2,3-Diaminophenazine . Deep eutectic solvents . Enzyme catalysis . Real samples . Milk . Urine . Kinetic assay . Inner filter effect
Introduction The o-phenylenediamine (OPD) is a significant chemical intermediate as well as a precursor for numerous heterocyclic compounds. Also, the industrial usage of OPD is very wide, especially to fabricate pesticide medicines, photosensitive resources, polymers, drugs, dyes, etc. [1]. A substantial quantity Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04256-0) contains supplementary material, which is available to authorized users. * Yaling Yang [email protected] 1
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan Province, China
of OPD found in human beings may cause liver damage, affect the digestive system and respiratory functions, and sometimes it may be prone to cance
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