Expression and analysis of zinc finger family gene in Lenzites gibbosa
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ORIGINAL PAPER
Expression and analysis of zinc finger family gene in Lenzites gibbosa Jun Zhang1,2 · Yujie Chi1 · Shuxuan Li1 · Jian Zhang1 · Jie Chen1
Received: 24 May 2019 / Accepted: 3 July 2019 © The Author(s) 2019
Abstract Zinc finger transcription factors play significant roles in the growth and development of plant and animal, but their function remains obscure in fungi. Lenzites gibbosa mycelia were extracted and sequenced by transcriptome analysis after growing on sawdust at different times to support mycelial growth of L. gibbosa in a nutrient matrix. Data bases used for analysis were the Kyoto encyclopedia of genes and genomes (KEGG) annotation, the cluster of orthologous groups of proteins (COG) and gene ontology (GO) annotation. Zinc finger class genes related to the growth and development of L. gibbosa were screened. GO annotation and enrichment analysis of differentially expressed genes were carried out. A total of 114.55 Gb Clean Data were obtained from the L. gibbosa transcriptome. The average Clean Data in each sample was 6.16 Gb. The relative efficiency of reads Project funding: The work was supported by grants from Special Funds for Sustainable Development of Science and Technology Platform for Fundamental Research Business Expenses of Central Universities (2572018CP05) and Key Projects of Universities for Foreign Cultural and Educational Experts Employment Plan in 2018 (T2018013). The online version is available at http://www.springerlink.com
between each sample and the reference genome was 88.5% to 91.4%. The COG analysis showed that most zinc finger protein genes were related to replication, recombination and repair function. GO enrichment analysis showed that the expressed genes involved in cellular process, cell part and binding. We identified seventy-two expressed genes including seven up-regulated genes and sixty-five down-regulated genes by applying DESeq2 data analysis software. By comparing the significantly expressed genes with KEGG database, 66 annotated sequences were obtained, and 35 primary metabolic pathways were annotated. Pathway enrichment analysis showed that differentially expressed genes were significantly enriched in protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis pathways. Gene_11750 and gene_5266 are highly correlated with the growth and development of L. gibbosa and are closely related to protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis pathway. According to gene functional analysis, seven important differentially expressed genes related to the growth and development of L. gibbosa were identified. Keywords Lenzites gibbosa · Differently expressed genes · The transcriptome · Zinc finger
Corresponding editor: Tao Xu. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11676-019-01044-2) contains supplementary material, which is available to authorized users. * Yujie Chi [email protected] 1
School of Forestry, Northeast Forestry University, Harbin 15004
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