Fungichromin production by Streptomyces sp. WP-1, an endophyte from Pinus dabeshanensis , and its antifungal activity ag
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BIOTECHNOLOGICAL PRODUCTS AND PROCESS ENGINEERING
Fungichromin production by Streptomyces sp. WP-1, an endophyte from Pinus dabeshanensis, and its antifungal activity against Fusarium oxysporum Cheng Peng 1 & Duopeng An 1 & Wen-Xin Ding 1 & Yuan-Xing Zhu 1 & Li Ye 1 & Jiyang Li 1 Received: 1 September 2020 / Revised: 20 October 2020 / Accepted: 31 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In this study, we isolated an endophytic Streptomyces sp. strain, WP-1, from surface-sterilized barks of Pinus dabeshanensis, an endangered Chinese plant. WP-1 showed strong antifungal activity against diverse pathogenic fungi, such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora infestan, and Candida albicans. Based on phylogenetic analyses, preliminary identification suggested that the WP-1 strain belonged to the genus Streptomyces. Column chromatogram and HPLC were employed to isolate the primary antifungal component from the culture medium of WP-1, and it was identified as the methylpentaene macrolide antibiotic, fungichromin (FC). In this study, for the first time, using in vitro bioassay studies, we revealed that FC strongly inhibited mycelial growth and conidia germination in Fusarium oxysporum. The median inhibitory concentration of FC was found to be 3.80 mg/L. The fermentation conditions of the WP-1 strain were further investigated to improve FC production. We found that supplementation of the synthetic medium with oils (soybean oil, oleic acid, and so on), particularly during the initial stage of fermentation, significantly increased the FC yield. Ammonium-trapping agent (magnesium phosphate) was used as an additive to increase FC yield to 5741.7 mg/L. It was 2.9-fold more as compared to the highest FC yield reported so far where Streptomyces padanus PMS-702 was used for FC production. Key points • Isolation and identification of a fungichromin-producing endophytic actinomycete WP-1 strain. • Fungichromin production was significantly improved via oils and ammonium-trapping agents addition. • Discovery of the antifungal activity of fungichromin against Fusarium oxysporum. Keywords Polyene macrolides . Fungichromin production . Endophytic actinomycete . Antifungal fermentation
Introduction Emerging fungal infections are being increasingly recognized as a threat to the ecosystem and human health across the world. These phytopathogenic fungi also affect the agriculture Cheng Peng and Duopeng An contributed equally to this work. Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s00253-02010996-z. * Jiyang Li [email protected] 1
Department of Biological Medicines, School of Pharmacy, Fudan University, Shanghai 201203, China
sector by infecting monoculture crops (Gurr et al. 2011). Fungal infection of healthy plants can be attributed to a variety of human activities, such as industrial pollution and resourcerich farming practices (Anderson et al. 2004). Although inadvertently, human activities are substanti
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