Streptomyces boluensis sp. nov., isolated from lake sediment
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ORIGINAL PAPER
Streptomyces boluensis sp. nov., isolated from lake sediment Ali Tokatli1,2 · Onder Idil3 · Aysel Veyisoglu4 · Hayrettin Saygin5 · Kiymet Guven6 · Demet Cetin7 · Nevzat Sahin5 Received: 21 February 2020 / Revised: 29 March 2020 / Accepted: 11 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain Y C537T, was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537T showed that it is closely related to the type strain of Streptomyces ziwulingensis F22T (97.9% sequence similarity), Streptomyces tauricus JCM 4837 T (97.7%) and Streptomyces beijiangensis NBRC 100044 T (97.6%). The cell wall of the strain contained LL-diaminopimelic acid and the cell-wall sugars were glucose, galactose and ribose. The major phospholipids of strain Y C537T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H6) and MK-9(H8). The major cellular fatty acids were iso-C16:0, iso-C14:0, anteiso-C15:0 and iso-C15:0. Consequently, strain YC537T is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces boluensis sp. nov. is proposed. The type strain is YC537T (= KCTC 39750 T = DSM 102303 T). Keywords Streptomyces boluensis sp. nov. · Polyphasic taxonomy · 16S rRNA gene
Introduction The genus Streptomyces was described by Waksman and Henrici in 1943, and at the time of writing, the genus comprised 800 species with validly published names (www.bacte rio.net/streptomyces.html). The genus Streptomyces was characterised by Gram-positive, aerobic and spore-forming actinomycetes, which have a GC-rich (69–73%) genome Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01901-3) contains supplementary material, which is available to authorized users.
(Williams et al. 1983). The genus Streptomyces includes LL-diaminopimelic acid in the cell-wall peptidoglycan (Kämpfer 2012). Streptomyces species synthesize a large number of different secondary metabolites, including antibiotics, antifungal compounds, antitumor agents enzymes, enzyme inhibitors and vitamins (Bérdy 2005; Goodfellow and Fiedler 2010; Katz and Baltz 2016). However, the rediscovery rate for known compounds from soil streptomycetes is increasing in recent years (Lewis 2013). Therefore, it has been directed to undiscovered habitats, mainly aquatic habitats, such as crater lake sediment (Ay et al. 2018), hot spring sediment (Duan et al. 2014), lake sediment (Xia et al. 2013), marine sediment (Veyisoglu and Sahin 2014), river (Zhao 4
Department of Medical Laboratory Techniques, Vocati
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