Genome-Wide Identification, Evolution, and Expression Profile of Aquaporin Genes in Coffea canephora in Response to Wate
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ORIGINAL ARTICLE
Genome-Wide Identification, Evolution, and Expression Profile of Aquaporin Genes in Coffea canephora in Response to Water Deficit Diliane Harumi Yaguinuma 1 & Tiago Benedito dos Santos 1 Luiz Gonzaga Esteves Vieira 1 & Alessandra Ferreira Ribas 1
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Silvia Graciele Hulse de Souza 2
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# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Aquaporins (APQs) belong to the major intrinsic protein superfamily and play a key role in the transport of water and other solutes across cell membranes. Cofe aquaporin genes assigned to five subfamilies including seven plasma membrane intrinsic proteins (PIP), 9 tonoplast intrinsic proteins (TIP), 11 NOD26-like intrinsic proteins (NIP), 3 small basic intrinsic proteins (SIP), and 3 X intrinsic proteins (XIP). Generally, the AQPs gene structure was conserved within each subfamily, with exon numbers ranging from one to five. The prediction of the aromatic/arginine selectivity filter (ar/R) and Froger’s positions indicated a noticeable difference in substrate specificity between subfamilies. Synteny analysis revealed high conservation of aquaporin genes in coffee. In silico expression analysis of the CcAQPs genes indicated that they were differentially expressed in various tissues. Members of CcPIPs and CcTIPs subfamilies were validated by real-time quantitative analysis in leaves of two genotypes of C. canephora with contrasting responses to water deficit (clone 14: drought-tolerant and clone 109A: drought-susceptible). Under severe water deficit, the relative expression of isoforms of both genes decreased in clone 14 compared with that under the irrigated condition, while clone 109A showed comparatively higher mRNA levels, with the exception of CcPIP1;2 in the stress condition. This study was the first to characterize and validate aquaporin genes in C. canephora in response to water deficit, and the findings may provide insights for biotechnological approaches to increase tolerance to drought. Keywords Robusta coffee . Water transporters . Abiotic stress . Gene expression
Introduction
Key Message •Aquaporin gene family conservation maintained in coffee plants. PIPs and TIPs isoforms expressed differentially in the contrasting genotypes of Coffea canephora in response to water deficit. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11105-020-01235-w) contains supplementary material, which is available to authorized users. * Alessandra Ferreira Ribas [email protected]; [email protected] 1
Faculty of Agronomy, Universidade do Oeste Paulista, Rodovia Raposo Tavares, km 572 – Bairro Limoeiro, Presidente Prudente, São Paulo 19067-175, Brazil
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Postgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense – UNIPAR, Praça Mascarenhas de Moraes, 4282, Umuarama, Paraná 87502-210, Brazil
Aquaporins (AQPs) belong to the major intrinsic protein (MIP) superfamily of integral membrane proteins present in bacteria, fungi, animals, and plants. These protein
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