Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
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Genomic Analysis of two NDM‑1 Providencia stuartii Strains Recovered from a Single Patient Amparo Hoard1 · Sabrina Montaña2 · Alessandro Moriano1 · Jennifer S. Fernandez1 · German M. Traglia2 · Cecilia Quiroga3 · Agustina Franchi4 · Emilia Cohen4 · Cecilia Corigliano4 · Marisa Almuzara4 · Maria Soledad Ramirez1 Received: 3 June 2020 / Accepted: 1 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In the last years, an increasing number of untreatable infections caused by drug-resistant microbes have impacted the health care system. Worldwide, infections caused by carbapenem-resistant (CR) Gram-negative bacilli have dramatically increased. Among the CR-Gram-negative bacilli, those producing carbapenemases, such as NDM-1, are the main concern. Different Enterobacterales harboring NDM-1 have been reported lately. Providencia stuartii, a member of the Morganellaceae family, is ubiquitous in the environment, but is also known to cause nosocomial infections. Here we describe the genomic analysis of two NDM-1- producing P. stuartii strains recovered from the same patient as well as other carbapenem resistant strains recovered from the same hospital. As a result of the genomic analysis thirteen resistance genes, including three to β-lactams (blaOXA-1, blaTEM-1, blaNDM-1), four to aminoglycosides (aphA6, aac(3)-IId, aac(2′)-Ia, aac(6′)-Ib-cr5), one to sulfonamides (sul1), two to chloramphenicol (catB3, catA3), one to rifampicin, one to bleomycin (ble), and one to tetracycline (tet(B)) were found. Moreover, a variety of mobile genetic elements, such as insertion sequences, plasmids and phage- related sequences, were found within P. stuartii genomes. The spread of carbapenem-resistant isolates remains a significant clinical and public health concern. Therefore, we considered that the detection of CR isolates is an essential step in addressing this problem.
Introduction Amparo Hoard, Sabrina Montaña have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02242-6) contains supplementary material, which is available to authorized users. * Maria Soledad Ramirez [email protected] 1
Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, CA, USA
2
Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia Y Bioquímica, Hospital de Clínicas José de San Martín, Buenos Aires, Argentina
3
Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas Y Tecnológicas, Instituto de Investigaciones en Microbiología Y Parasitología Médica (IMPAM), Facultad de Medicina, Paraguay 2155‑ C1121ABG Buenos Aires, Argentina
4
Laboratorio de Bacteriologia, Hospital Interzonal de Agudos Eva Perón, Provincia de Buenos Aires, San Martín, Argentina
Carbapenem antibiotics are generally considered to be the last option in the treatment of patients with severe bacterial infections.
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