Human gut microbiota and its possible relationship with obesity and diabetes
- PDF / 495,762 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 86 Downloads / 167 Views
ORIGINAL ARTICLE
Human gut microbiota and its possible relationship with obesity and diabetes Nima Mohammadzadeh 1,2 & Shabnam Razavi 2,3 & Zahra Hadi 4 & Mohammadreza Kermansaravi 5,6 & Shahin Boloori 7 & Ali Kabir 8 & Mohammad E. Khamseh 9 Received: 24 August 2019 / Accepted: 10 October 2020 # Research Society for Study of Diabetes in India 2020
Abstract Background Obesity and diabetes are public health problems that are leading causes of death in the world. Recent surveys suggest that there is a relationship between diabetes and bacterial residents of the gastrointestinal tract. Objective This case-control study was designed to evaluate the composition of the gut microbiota in patients with type 2 diabetes (T2DM) and obesity compared to the healthy people. Methods A total of 91 adult subjects (25 patients diagnosed with T2DM, 48 obese patients, and 18 healthy individuals) were included in the study. The gut microbiota composition was investigated by quantitative real-time polymerase chain reaction (qPCR) method using bacterial 16S rRNA gene. Results The frequency of all bacterial species in the obese group compared to the control group have significantly changed (p < 0.05) except Bacteroides fragilis, whereas the level of bacterial composition was not changed significantly (p > 0.05) in the diabetic patients versus the control ones, except for Bacteroides phylum and Lactobacillus spp. Moreover, the mean body mass index (BMI) in control, T2DM, and obese groups were 24.28 ± 3.00, 26.83 ± 3.29, and 44.65 ± 3.73, respectively. Our analysis showed a positive correlation between diabetic patients plus obese ones and the number of bacteria (p < 0.05). Conclusions To sum up, these findings show that specific changes in microbial community composition are associated with T2DM and obesity. More extensive, our survey suggests that modulation of the microbiome warrants further investigation as a potential therapeutic strategy for metabolic diseases. Keywords Gut microbiota . Obesity . Type 2 diabetes mellitus . Real-time PCR
Introduction Over 10–100 trillion microbes colonize in each part of human body, including the skin, vagina, oronasopharyngeal cavity, and of course gastrointestinal tract [1, 2]. The gastrointestinal microbiota generally refers to the microbial composition in the * Shabnam Razavi [email protected] 1
Faculty of Science, Department of Microbiology, Shahid Beheshti University, Tehran, Iran
2
Faculty of Medicine, Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran
3
Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran
4
Department of Microbiology, School of Basic Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
gut, which contains various types of microorganisms such as bacteria, viruses, archaea, fungi, as well as phages [3]. Among all different bacterial species, which colonized in human guts, five phyla are most abundant, including Firmicutes, Bacteroidetes, Verrucomicrobia, Actinobacteria, and Proteobacteria [3]. T
Data Loading...
