Identification and characterization of PaGL1-like genes from Platanus acerifolia related to the regulation of trichomes
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Identification and characterization of PaGL1‑like genes from Platanus acerifolia related to the regulation of trichomes Yanping Zhang1,2 · Changsheng Shao1,2 · Zhiru Bao1,2 · Fangfang Cai1,2 · Haofei Zhu1,2 · Jiaqi Zhang1,2 · Manzhu Bao1,2 Received: 23 October 2019 / Accepted: 26 June 2020 © Springer Nature B.V. 2020
Abstract Key Message Two PaGL1-like genes were identified in London plane and functional in Arabidopsis, moreover, may play an important role in the regulation of trichome development in London plane. Abstract Trichome development is governed by a complex regulatory network. In Arabidopsis, subgroup 15 of the R2R3 MYB transcription factor family, which includes GLABRA1 (GL1), is involved in trichome development. In this study, we isolated and characterized two PaGL1-like genes from London plane (Platanus acerifolia). Sequence alignment and phylogenetic analysis indicated that these PaGL1-like genes are homologous to AtGL1. Quantitative real-time PCR (qRT-PCR) analysis showed that PaGL1-like1 was expressed in all of the tested organs taken from adult London plane trees, including trichomes, petioles after trichome removal, stems after trichome removal, and leaves after trichome removal, and also in the roots, cotyledons, hypocotyls and true leaves of seedlings. By contrast, the PaGL1-like2 was expressed only in the trichomes and leaves after trichome removal from adult trees, and in the cotyledons and true leaves of seedlings. Overexpression of PaGL1-like genes caused trichome abortion when transferred into wild type Arabidopsis and promoted trichome formation in the gl1 mutant. The expression profiles of some trichome-related genes were changed in transgenic Arabidopsis lines, and yeast two-hybrid analysis indicated that PaGL1-like proteins can directly interact with trichome-related bHLH proteins from both P. acerifolia and Arabidopsis. These results suggest that PaGL1-like genes are functional in Arabidopsis and may play an important role in the regulation of trichome development in London plane. Keywords London plane · Protein interaction · R2R3 MYB transcription factor · Trichome regulation
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01028-5) contains supplementary material, which is available to authorized users. * Jiaqi Zhang [email protected] * Manzhu Bao [email protected] 1
Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430070, People’s Republic of China
2
Trichomes are hair-like structures which are widely distributed on the epidermal surfaces of many plant species and may act as barriers to protect plants from herbivores, UV irradiation and excessive transpiration (Johnson 1975; Mauricio and Rausher 1997; Werker 2000; Maes et al. 2008). Accor
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