Identification of differential expressed proteins and establishing a defense proteome of sugarcane in response to Collet
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ORIGINAL ARTICLE
Identification of differential expressed proteins and establishing a defense proteome of sugarcane in response to Colletotrichum falcatum infection Velusamy Ganesh Kumar 1 & Rasappa Viswanathan 1 & Palaniyandi Malathi 1 & Amalraj Ramesh Sundar 1 & Chandrasekaran Naveen Prasanth 1 & M. Nandakumar 1 Received: 2 January 2018 / Accepted: 16 May 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract Defense proteome of sugarcane in response to red rot pathogen Colletotrichum falcatum was established for the first time upon analyzing the differentially expressed proteins of resistant and susceptible cultivars through two dimensional gel electrophoresis (2-DE) and mass spectrometry. The proteomes of red rot resistant (Co 93009) and susceptible varieties (CoC 671) showed significant differences in their protein expression after pathogen inoculation. The resistant variety showed a dynamic activation and regulation of cellular components upon infection from the pathogen, whereas the response was submissive in the susceptible variety. A total of 136 differentially expressed proteins were characterized by MALDI-TOF and the identified proteins were grouped into seven broad categories based on their putative functions. The major portion of the up-regulated proteins belonged to signal transduction, transcription/ transcription factors, metabolism, defense/ stress and retroelements. Among the downregulated proteins, majority belonged to metabolism, cell growth and development, followed by signal transduction and transcription factors. The important proteins identified by MALDI-TOF analyses were of those involved in various defense/ cellular functions such as cyclic nucleotide gated ion channel protein, callose synthase, R2R3-MYB transcription factor MYB6, WRKY transcription factor, putative p-coumarate 3-hydroxylase, PISTILLATA-like protein, flagellar associated protein, class III HDZip protein HDZ34, calcium dependent protein kinase, DNAJ heat shock protein, polyphenoloxidase, cytokinin oxidase 3, NBARC domain containing protein, NADH dehydrogenase, UDP glucose:flavonoid 3-O-glucosyltransferase, ACC synthase and putative disease resistance protein. The isolation and characterization of differentially expressed proteins would help us to understand the complex host-pathogen interaction at molecular level during the course of disease development/ progression. Keywords Sugarcane . Red rot . Colletotrichum falcatum . Defense proteome
Introduction Red rot of sugarcane caused by Colletotrichum falcatum Went is a serious threat to sugarcane cultivation in India and other South Asian countries like Pakistan, Bangladesh and Nepal. Growing red rot resistant varieties has become the rule to sustain the sugarcane cultivation in India. Besides yield and quality, it is imperative to give equal importance to incorporate red rot resistance while breeding sugarcane varieties,
* Rasappa Viswanathan [email protected] 1
ICAR-Sugarcane Breeding Institute, 641007 Coimbatore, Tamil Nadu, India
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