Insight to the ancestral relations and varietal diversity of Indian tea [ Camellia sinensis (L.) Kuntze] through plastid
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RESEARCH ARTICLE
Insight to the ancestral relations and varietal diversity of Indian tea [Camellia sinensis (L.) Kuntze] through plastid and nuclear phylogenetic markers Anjan Hazra . Pradosh Mahadani . Subhanwita Das . Senjuti Bhattacharya . Rakesh Kumar . Chandan Sengupta . Sauren Das
Received: 18 April 2020 / Accepted: 16 September 2020 Ó Springer Nature B.V. 2020
Abstract Traces of genetic introgression from wild donors that occurred during the origin trajectory of a crop would be concealed in the existing gene pool of its cultivated varieties. Dissection of signature motifs from wild relatives and diverse genotypes of a crop, not only can elucidate the probable ancestry but also offer a potential tool to authenticate varieties with a particular phenotype. We selected twenty-two distinct genotypes representing diverse commercial tea germplasms of both assamica and sinensis lineage to trace Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-01022-2) contains supplementary material, which is available to authorized users. A. Hazra S. Das (&) Agricultural and Ecological Research Unit, Indian Statistical Institute, 203, B. T. Road, Kolkata 700108, India e-mail: [email protected]
genetic signatures of their wild ancestors. A total of 176 sequences were generated from eight coding and non-coding loci belonging to both plastid and nuclear genome of the commercial tea accessions and compared with corresponding homologous loci from various wild Camellia species. The concatenated multi-locus tree of the selected cultivars and the allied species was constructed to examine phylogenetic relatedness among and within the species. Present study uncovered several signature motifs that carry evidence of multiple hybridization events during the origin trajectory of the cultivated tea plant. Identified genotype-specific indels of chloroplast and nuclear non-coding regions might imitate their varietal origin. R. Kumar Darjeeling Tea Research and Development Center, Kurseong, West Bengal 734203, India
A. Hazra C. Sengupta Department of Botany, University of Kalyani, Nadia 741235, India P. Mahadani Bioinformatics Center, National Tea Research Foundation, Tea Park, Siliguri 735135, India S. Das S. Bhattacharya Department of Molecular Biology and Biotechnology, University of Kalyani, Nadia 741235, India
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Genet Resour Crop Evol
Keywords Camellia Intergenic spacer Plant systematics Tea Varietal authentication
Introduction The tea plant was introduced sometimes around 2700 BC into domestic practices and today this globally consumed beverage attends almost 2 billion cups regularly (Drew 2019). Sources of all commercial teas produced in the world belong to three distinct intraspecific lineages, referred to as small-leaved China variety [Camellia sinensis (L.) Kuntze var. sinensis], large-leaved Assam variety [Camellia sinensis var. assamica (J. W. Mast.) Kitam.] and an intermediate type or Cambod variety (Chen et
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