Ionic liquid synergistic effect between preparation of hybrid supports and immobilization of lipase applied to esters pr
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Ionic liquid synergistic effect between preparation of hybrid supports and immobilization of lipase applied to esters production Sílvia R. S. Martins1 · Suellen M. S. Andrade1 · Alini T. Fricks1,2 · Álvaro S. Lima1,2 · Daniel P. Silva3 · Renan T. Figueiredo1,2 · Lisiane S. Freitas3 · Rebeca Y. Cabrera‑Padilla4 · Cleide M. F. Soares1,2 · Ranyere L. Souza1,2 Received: 7 January 2020 / Accepted: 13 November 2020 © Akadémiai Kiadó, Budapest, Hungary 2020
Abstract The present work aims to evaluate the synergistic effect of imidazolium-based ionic liquids (IL) in the preparation and immobilization of Burkholderia cepacia lipase in a new hybrid support of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and silica. In addition, these biocatalysts were applied in transesterification reactions of crude coconut oil for the production of ethyl esters. Lipase immobilized on hybrid support with [ C4min][NTf2] or [C6min][NTf2] ionic liquid exhibited nearly ≈ 1.6-fold immobilization yield greater than IB-Control. The use of IL during the preparation of the hybrid support promoted changes in its morphological structure as presented by B.E.T. and FTIR. Thermogravimetric analyzes were used to characterize the prepared derivatives and support the results of immobilization yields and the synergic effect of IL used. Transesterification reactions applying biocatalysts showed that the conversion maximum in esters (59%) was found under conditions of molar ratio 1:7 (oil:ethanol) in 72 h of reaction. These results were obtained using [C4min][NTf2] during the preparation of the hybrid support for the immobilization of Burkholderia cepacia lipase, thus showing the potential of these new biocatalysts. Keywords Biocatalysts · Hybrid support · Immobilization · Ionic liquids · Transesterification
Introduction Conventional catalysis promotes the accumulation of toxic waste and presents a high energy expenditure [1]. In order to minimize these adversities, chemical catalysis can be replaced by biological catalysts, such as, the proposed methods with Burkholderia cepacia lipase, which allow for the formation of purer products meanwhile demanding lower energy consumption [2]. Furthermore, the biocatalysts are * Ranyere L. Souza [email protected] 1
Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia 49032‑490, SE, Brasil
2
Instituto de Tecnologia E Pesquisa, Av. Murilo Dantas 300 ‑ Prédio do ITP ‑ Farolândia, 49032‑490 Aracaju, SE, Brasil
3
Centro de Ciências Exatas E Tecnologia, Universidade Federal de Sergipe, Av. Marechal Rondon s/n, Jardim Rosa Elze, 49100‑000 São Cristóvão, SE, Brasil
4
Instituto de Química, Universidade Federal de Mato Grosso Do Sul, Avenida Senador Filinto Müller 1555, Cidade Universitária, Campo Grande 79074‑460, MS, Brasil
selective and present high versatility, being able to perform specific biocatalytic reactions, acting under mild conditions of temperature and pressure [3, 4]. Although enzymes have excellent perspectives as catalysts, their industrial application is not immediately
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