Isolation and characterization of 18 microsatellite loci in the Chinese Noctule bat, Nyctalus plancyi
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MICROSATELLITE LETTERS
Isolation and characterization of 18 microsatellite loci in the Chinese Noctule bat, Nyctalus plancyi Hao Liu • Yujie Ran • Hongyan Shi Xiaodong Jia • Lin Zhao
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Received: 4 October 2014 / Accepted: 15 October 2014 / Published online: 22 October 2014 Ó Springer Science+Business Media Dordrecht 2014
Abstract Nyctalus plancyi is an endemic species restricted to China. Its population is declining drastically, because of habitat loss. Here, we describe 18 microsatellite loci that were isolated from N. plancyi. All loci were highly polymorphic. The number of alleles per locus ranged from 5 to 11. The observed and expected heterozygosity values per locus ranged from 0.391 to 0.957 and from 0.645 to 0.910, respectively. Two loci showed significant deviations from Hardy–Weinberg equilibrium, while no significant linkage disequilibrium was found between any pair of loci. These informative microsatellite markers will be a powerful molecular tool for studying population structure and mating system of N. plancyi. Keywords Nyctalus plancyi Microsatellite loci Polymorphism The Chinese Noctule bat (Nyctalus plancyi, synonyms: N. velutinus) is an endemic species restricted to China (Smith and Xie 2009; Simmons 2005). It typically roosts in eaves of a roof, ceilings and crevices of buildings. However, its population is declining drastically, probably because of
Electronic supplementary material The online version of this article (doi:10.1007/s12686-014-0364-8) contains supplementary material, which is available to authorized users. H. Liu H. Shi (&) X. Jia L. Zhao Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, No. 166, Mianxing West Road, Gaoxin District, Mianyang 621000, China e-mail: [email protected] Y. Ran Animal Behaviour Research Group, College of Life Science, Central China Normal University, 152 Luoyulu, Hongshan District, Wuhan 430079, China
habitat loss (Shi et al. 2008). Little is known about the mating behavior, population structure and gene flow within and between populations of this species. In recent years, microsatellites have become one of the most popular molecular marker system used in researches of population structure and mating behavior. However, no microsatellite primers specific for N. plancyi have been reported to date. Here, we describe 18 microsatellite loci that were isolated from N. plancyi, in order to study population structure, genetic diversity and mating system of this species. N. plancyi wing punches samples were collected from one wild population in the campus of Xihua Normal University in Sichuan Province, China. Genomic DNA was isolated according to standard phenol–chloroform protocols or using a TIANamp Genomic DNA Kit (TIANGEN). The (AGAT)n microsatellite-enriched libraries were constructed based on the protocol described by Zhao et al. (2012). Approximately 480 colonies were screened by PCR amplification, and 117 positive clones were sequenced on an ABI XL 3730 DNA Analyzer (Applied Biosystems). Fifty-sev
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