Optimization and scale-up of laccase production by Bacillus sp. BAB-4151 isolated from the waste of the soap industry
- PDF / 1,894,254 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 97 Downloads / 229 Views
ORIGINAL ARTICLE
Optimization and scale‑up of laccase production by Bacillus sp. BAB‑4151 isolated from the waste of the soap industry T. Deepa1 · A. K. Gangwane1 · R. Z. Sayyed2 · H. P. Jadhav2 · Mehjabeen3 Received: 13 June 2020 / Revised: 19 September 2020 / Accepted: 1 October 2020 © Society for Environmental Sustainability 2020
Abstract Laccase is also known as the blue copper oxidase enzyme. Owing to its ability to oxidizes a wide variety of phenolic and non-phenolic substrates, this enzyme has many biotechnological applications. It is produced by plants, fungi, and a wide variety of bacteria. However, bacterial laccases have been studied more due to their merits over other sources. Production of bacterial laccase at the commercial level demands high yielding cultures and optimization of physicochemical parameters for more yields in a shorter period. Hence, the present research was aimed to isolate the potent laccase producing bacteria and to optimize cultural conditions for higher production of laccase. In this study, six laccase positive bacterial cultures were isolated from the waste of the soap industry in Kadi, Gujarat, India. One isolate later identified as Bacillus sp. BAB4151 produced copious amounts of laccase. The production process of laccase was optimized at shake flask level by using one variable at a time (OVAT) method and scaled-up to 5 L capacity bioreactor. The isolate produced a higher yield of laccase under diverse physicochemical and nutritional conditions. The OVAT approach resulted in 2.9 folds improvement in the yield of laccase, which further increased by 1.4 fold during scale-up at a laboratory-scale bioreactor. The ability of the organism to produce laccase over of range of physico-chemical conditions and to retain the productivity during scale up of the process at bioreactor level suggests that the organism can be of great commercial significance. Keywords ABTS · Bacillus sp. BAB-4151 · Extracellular-laccase · Guaiacol · Ribotyping · Shake flask · Optimization · Tannic-acid
Introduction Laccase is an enzyme that belongs to the blue copper oxidase group. This enzyme has been extensively studied due to its many useful properties such as its ability to reduce the molecular oxygen into water, wide substrate specificity (Thurston 1883), less fermentation time, and more stability at a broad scale of pH and temperature (Zhang et al. 2019). These properties of laccase make it a suitable enzyme for a wide range of applications. These applications include * R. Z. Sayyed [email protected] 1
Faculty of Applied Sciences, Parul University, Vadodara, Gujarat 391760, India
2
Department of Microbiology, PSGVP Mandal’s Arts, Science, and Commerce College, Shahada, Maharashtra 425409, India
3
Department of Economics, Periyar University, Salem 636011, India
bioremediation of industrial waste (Mendoza et al. 2011); degradation of lignin, phenolic substrate and dyes; bioremediation and neutralization of toxic chemicals in the effluent (Mayer and Staples 2002); development of biosens
Data Loading...
