Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glaci
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ORIGINAL PAPER
Alkaline lipase production by novel meso‑tolerant psychrophilic Exiguobacterium sp. strain (AMBL‑20) isolated from glacier of northeastern Pakistan Muhammad Talha Yasin1 · Yasir Ali2 · Khurshid Ahmad2 · Abdul Ghani2 · Kinza Amanat3 · Muhammad Mudassir Basheir2 · Muhammad Faheem2 · Saddam Hussain2 · Bashir Ahmad2 · Adil Hussain4 · Syed Ali Imran Bokhari2 Received: 3 January 2020 / Revised: 15 November 2020 / Accepted: 19 November 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The isolated bacterium was identified as Exiguobaterium sp. strain AMBL-20T on the basis of morphological, biochemical, and phylogenetic analysis of 16S rRNA sequences with GenBank accession number MW229267. The bacterial strain was further screened for its lipolytic activity, biosynthesis, and characterization by different parameters with the aim of maximizing lipase activity. Results showed that 2% Olive oil, 0.2% peptone at 25 °C, pH 8, and 24 h of incubation time found optimal for maximum lipase production. The lipase enzyme was partially purified by ammonium sulphate precipitation and its activity was standardized at pH 8 under 30 °C temperature. The enzyme showed functional stability over a range of temperature and pH. Hence, extracellular alkaliphilic lipase from Exiguobacterium sp. is a potential candidate with extraordinary industrial applications, particularly in bio-detergent formulations. Keywords Exiguobaterium sp. · Lipase · Optimization · Submerged fermentation · Bio-detergent formulation · Gilgitbaltistan
Introduction Lipase (E.C.3.1.1.3) is recognized as an industrially important enzyme considering wide-ranging applications in food, dairy, detergent, leather, and oil processing industries. Owing to its hydrolytic potential, lipase has been explored Communicated by Erko Stackebrandt. * Muhammad Talha Yasin [email protected] 1
Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan
2
Department of Biological Sciences, International Islamic University Islamabad, Islamabad 44000, Pakistan
3
Department of Microbiology, University of Agriculture, Faisalabad 38000, Pakistan
4
Department of Biotechnology, University of Okara, Okara 56130, Pakistan
for biotechnological applications i.e. biodiesel production, biopesticides, flavoring, biopolymers, oil, and fat hydrolysis (Vitorino et al. 2017). Lipases comprehend versatile characteristics of catalyzing reactions such as esterification, hydrolysis, alcoholysis, transesterification, and are widely employed in industry (Ferreira-Leitão et al. 2017). Structurally, a mobile subdomain is present in most lipases called lid that regulates the access of substrate to the catalytic site (Bor
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