Optimization of Extraction Conditions for Glycosaminoglycan from Urechis unicinctus by Response Surface Methodology
The enzyme dosage, enzymolysis time, and alcohol precipitation concentration as independent variables and the extraction rate as the response value, the extraction technology of glycosaminoglycan from Urechis unicinctus was optimized via response surface
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Optimization of Extraction Conditions for Glycosaminoglycan from Urechis unicinctus by Response Surface Methodology Chunying Yuan, Xu Han, Qingman Cui and Ping Liu Abstract The enzyme dosage, enzymolysis time, and alcohol precipitation concentration as independent variables and the extraction rate as the response value, the extraction technology of glycosaminoglycan from Urechis unicinctus was optimized via response surface methodology on the basis of single-factor experiments. The extraction rate of glycosaminoglycan was affected in the following order: concentration of alcohol precipitation [ enzymolysis time [ enzyme dosage. The optimal conditions for the glycosaminoglycan extraction were as follows: alcohol precipitation concentration 75 %, enzymolysis time 4 h, and enzyme dosage 0.4 %.
Keywords Urechis unicinctus Glycosaminoglycan Optimization of extraction conditions Response surface methodology
126.1 Introduction Glycosaminoglycans, such as heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, hyaluronic acid, and keratan sulfate, are polysaccharides containing uronic acid and hexosamine. They not only exhibit anticoagulant, hypolipidemic, antitumor, and antiviral activities, but also enhance the body’s immune system [1, 2]. Glycoproteins or glycosaminoglycans have been extracted from Argopecten irradians, Ruditapes philippinarum, Sinonovacula constricta, Scapharca subcrenata, Mactra veneriformis, and Bullacta exarata et al., and some of their activities have been investigated [3–10]. Urechis unicinctus is a species of marine spoon worms, also known as sea intestines, mainly distributed in the Yellow Sea, Bohai Coast. It C. Yuan (&) X. Han Q. Cui P. Liu Key Laboratory for Marine Chemistry and Resource, College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China e-mail: [email protected]
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 250, DOI: 10.1007/978-3-642-37922-2_126, Ó Springer-Verlag Berlin Heidelberg 2014
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is rich in protein and many essential amino acids. Research had shown that the polypeptide extracted from U. unicinctus exhibited a certain antitumor activity and improved the immune function of mice, and the fibrinolytic enzyme extracted from the same species had antithrombotic effects [11, 12]. In order to utilize fully the quality resources, we optimized the extraction methods of glycosaminoglycan from U. unicinctus.
126.2 Materials and Methods 126.2.1 Materials U. unicinctus were purchased from farmers’ markets in Tianjin Tanggu. Trypsin (enzyme activity, 250 U/g; optimum temperature, 50 °C; optimum pH of 8.0) was purchased from Beijing Solarbio Science & Technology Co., Ltd. A Ds-1 high-speed organization broken machine, a SHA-C water bath oscillator, a T6 new century UV/Vis spectrophotometer, a TGL-16LM high-speed refrigerated centrifuge, a 99-1 magnetic stirrer, a RE-3000 rotary eva
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