OsJAZ9 overexpression modulates jasmonic acid biosynthesis and potassium deficiency responses in rice
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OsJAZ9 overexpression modulates jasmonic acid biosynthesis and potassium deficiency responses in rice Ajit Pal Singh1 · Bipin K. Pandey1,2 · Poonam Mehra1 · Thierry Heitz3 · Jitender Giri1 Received: 4 March 2020 / Accepted: 6 August 2020 © Springer Nature B.V. 2020
Abstract Key message Enhanced bioactive JA (JA-Ile) accumulation in OsJAZ9 overexpressing rice helps plants tolerate K deficiency. Abstract Potassium (K) represents up to 10% of the plant’s total dry biomass, and its deficiency makes plants highly susceptible to both abiotic and biotic stresses. K shortage results in the inhibition of root and shoots growth, but the underlying mechanism of this response is unclear. Our RNA-Seq and qPCR analysis suggested leading roles for JA pathway genes under K deficiency in rice. Notably, K deficiency and JA application produced similar phenotypic and transcriptional responses. Here, we integrated molecular, physiological and morphological studies to analyze the role of OsJAZ9 in JA homeostasis and K deficiency responses. We raised OsJAZ9 over-expression, knockdown, transcriptional reporter, translational reporter and C-terminal deleted translational reporter lines in rice to establish the role of JA signaling in K ion homeostasis. JA profiling revealed significantly increased JA-Ile levels in OsJAZ9 OE lines under K deficiency. Furthermore, we established that OsJAZ9 overexpression and knockdown result in K deficiency tolerance and sensitivity, respectively, by modulating various K transporters and root system architecture. Our data provide evidence on the crucial roles of OsJAZ9 for improving K deficiency tolerance in rice by altering JA levels and JA responses. Keywords Abiotic stress · Potassium deficiency · JAZ repressor · Root system architecture · Lateral roots
Introduction Rice is a mainstay for global food security. The two-third population of the world is dependent on rice as a staple food crop. Soil nutrient deficiency is one of the primary limitations of rice production. Notably, Potassium (K) is one of the most important and abundant macronutrients for plants, Ajit Pal Singh and Bipin K. Pandey have equal contribution. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01047-2) contains supplementary material, which is available to authorized users. * Jitender Giri [email protected] 1
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India
2
Plant and Crop Science Division, School of Biosciences, University of Nottingham, Nottingham, UK
3
Institut de Biologie Moléculaire des Plantes (IBMP) du CNRS, Université de Strasbourg, Strasbourg, France
which can comprise up to 10% of the plant’s total dry weight (Leigh and Wyn Jones 1984). Its physiological functions fall into two categories; the first requires a high and relatively stable concentration of K+ to regulate the osmotic potential of the cell and activation of many enzymes involved in respiration and photosynthesis ( K+ act as a cofactor
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