Plant Respiration and Internal Oxygen Methods and Protocols
This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation
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Kapuganti Jagadis Gupta Editor
Plant Respiration and Internal Oxygen Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Respiration and Internal Oxygen Methods and Protocols
Edited by
Kapuganti Jagadis Gupta National Institute of Plant Genome Research, New Delhi, Delhi, India
Editor Kapuganti Jagadis Gupta National Institute of Plant Genome Research New Delhi, Delhi, India
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7291-3 ISBN 978-1-4939-7292-0 (eBook) DOI 10.1007/978-1-4939-7292-0 Library of Congress Control Number: 2017951627 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Respiration is a very important biochemical process. Aerobic respiration in plants, as in all eukaryotes, involves the oxidation of organic substrates such as carbohydrates, lipids, proteins, amino acids, and organic acids, leading to the production of ATP and the release of CO2 and H2O. As side products, several reactive oxygen and nitrogen species are also produced during respiration and they play important roles in cellular signaling. Respiratory metabolism can also generate intermediates that are required for the biosynthesis of amino acids, nucleic acids, fatty acids, and antioxidants. Respiration involves glycolysis, the pyruvate dehydrogenase complex, the TCA cycle, and the mitochondrial electron transport chain. In glycolysis energy is released for the synth
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