Prevalence of Helicobacter pylori babA , oipA , sabA , and homB genes in isolates from Chinese patients with different g
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ORIGINAL INVESTIGATION
Prevalence of Helicobacter pylori babA, oipA, sabA, and homB genes in isolates from Chinese patients with different gastroduodenal diseases Qiaoyun Zhao1,2,3 · Conghua Song1,2,3,5 · Ke Wang4 · Donghong Li4 · Yang Yang1,2,3 · Dongsheng Liu2,3 · Le Wang2,3 · Nanjin Zhou4 · Yong Xie1,2,3 Received: 30 January 2020 / Accepted: 16 March 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Disease outcome is associated with virulence factors of Helicobacter pylori (H. pylori), which are partially attributed to the outer membrane protein (OMP). This study aimed to investigate the correlation between the four OMP genes (babA, oipA, sabA, and homB) and gastroduodenal diseases. One hundred and seventy-seven H. pylori strains were isolated from Chinese patients with different gastroduodenal diseases (49 chronic gastritis, 19 gastric ulcer, 33 gastric cancer, and 76 duodenal ulcer), 94 of which contained pathological information (41 superficial gastritis, 24 intestinal hyperplasia, and 29 gastric adenocarcinoma). The full-length amplification of babA, oipA, sabA, and homB genes was acquired and sequenced. Then, the genetic polymorphism was analyzed to compare with the reference strains from the GenBank database. Functional status and cluster analysis were also performed to evaluate the impact of genetic polymorphism on disease outcome. The prevalence of babA, oipA, sabA, and homB genes were 91.5%, 100%, 94.0%, and 95.5%, respectively. The four OMP genes were characterized by genetic polymorphism and in the status of positive selection (Ka/Ks> 1). The proportion of strains with functional status on for oipA and sabA gene was 100% and 76.2%, respectively. The sequences of four OMP genes were mainly clustered together with the East Asian references. The four OMP genes were not different in patients with gastroduodenal diseases and pathologic changes (P > 0.05). H. pylori babA, oipA, sabA, and homB genes were common in the Chinese populations, but did not seem to be involved in the development of gastroduodenal diseases. Keywords Helicobacter pylori · Outer membrane protein · Gastroduodenal diseases · Gene sequence · Genetic polymorphism · Phylogeny
Introduction Helicobacter pylori (H. pylori) is a slow-growing, microaerophilic, Gram-negative, spiral-shaped and flagellated bacterium, which colonizes the mucus layer of the gastric Edited by: Volkhard A. J. Kempf. Qiaoyun Zhao and Conghua Song are joint first authors of this paper. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00430-020-00666-2) contains supplementary material, which is available to authorized users. * Yong Xie [email protected] Extended author information available on the last page of the article
epithelium [1]. Approximately one-half of the global human population was colonized by this organism, especially in developing countries [2]. Although Helicobacter pylori (H. pylori) infection can induce inflammatory responses of host cells, the majority of infec
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