Proteomics in Systems Biology Methods and Protocols
This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology. Written in the highly successful Methods in
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Jörg Reinders Editor
Proteomics in Systems Biology Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Proteomics in Systems Biology Methods and Protocols
Edited by
Jörg Reinders Institute of Functional Genomics, University of Regensburg, Regensburg, Germany
Editor Jörg Reinders Institute of Functional Genomics University of Regensburg Regensburg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3339-6 ISBN 978-1-4939-3341-9 (eBook) DOI 10.1007/978-1-4939-3341-9 Library of Congress Control Number: 2015957400 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Proteomics techniques have constantly been developed further through the last decade and have been applied successfully for all kinds of samples and biological or medical questions. Nowadays, they are established methods in many research labs, and proteomic studies can be accomplished with good reliability and coverage on a routine basis. Therefore, proteomics can be used as a powerful tool in functional genomics and systems biology studies. Current challenges are thus the implementation of proteomic analyses in these comprehensive studies. This applies for both sample generation and preparation to ensure consistency over several levels of analyses like genomics, transcriptomics, and metabolomics and integration of the multilevel data to generate biological knowledge. This book gives an overview of contemporary quantitative proteomics methods and data interpretation approaches and also gives examples of how to i
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