Quantitative and simultaneous detection of two inflammation biomarkers via a fluorescent lateral flow immunoassay using
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ORIGINAL PAPER
Quantitative and simultaneous detection of two inflammation biomarkers via a fluorescent lateral flow immunoassay using dual-color SiO2@QD nanotags Xingsheng Yang 1,2 & Xiaoxian Liu 1,2 & Bing Gu 3,4 & Haifeng Liu 1,2 & Rui Xiao 2 & Chongwen Wang 1,2,3
& Shengqi Wang
2
Received: 10 May 2020 / Accepted: 7 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract An on-site detection strategy is reported based on dual-color SiO2@quantum dot (QD)–integrated lateral flow immunoassay (LFA) strip to realize the quantitative and simultaneous detection of C-reactive protein (CRP) and procalcitonin (PCT) in serum. The dual-color SiO2@QD nanotags with monodispersity and excellent luminescence were synthesized using polyethyleneiminemediated electrostatic adsorption of dense red CdSe/ZnS-COOH (excitation/emission 365/625 nm) or green CdSe/ZnS-COOH (excitation/emission 365/525 nm) QDs on the surface of 180 nm SiO2 spheres and were conjugated with anti-PCT and anti-CRP monoclonal antibodies, as stable and fluorescent-enhanced QD nanotags in the LFA system. The use of SiO2@QDs with two different fluorescent signals caused the sensitivity and specificity of the multiplex LFA system. As a result, the proposed assay provided a wide logarithmic determination range with a CRP quantitative range of 0.5–103 ng/mL and PCT quantitative range of 0.05–103 ng/mL. The limits of detection (LODs) of CRP and PCT reached 0.5 and 0.05 ng/mL, respectively. The SiO2@QDbased LFA showed great potential as rapid detection tool for the simultaneous monitoring of CRP and PCT in serum sample. Keywords Dual-color SiO2@QDs . Fluorescent lateral flow immunoassay . C-reactive protein . Procalcitonin
Introduction Xingsheng Yang, Xiaoxian Liu and Bing Gu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04555-6) contains supplementary material, which is available to authorized users. * Rui Xiao [email protected] * Chongwen Wang [email protected] * Shengqi Wang [email protected] 1
College of Life Sciences, Anhui Agricultural University, Hefei 230036, People’s Republic of China
2
Beijing Institute of Radiation Medicine, Beijing 100850, People’s Republic of China
3
Medical Technology Institute of Xuzhou Medical University, Xuzhou 221004, People’s Republic of China
4
Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, People’s Republic of China
Sepsis is a life-threatening systemic inflammatory response caused by the over-reaction of the body to infections from pathogenic microorganisms [1–3]. Sepsis affects more than 30 million people each year around the world; the mortality among patients in the intensive care unit has reached 40% [4]. The timely and accurate diagnosis of sepsis is difficult for clinicians, due to its clinical symptoms being usually atypical, heterogeneous, and nonspecific [5, 6]. Conventional diagnostic methods mainly include blood cultures, bloo
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