SELDI-TOF Mass Spectrometry Methods and Protocols
SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been wid
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
SELDI-TOF Mass Spectrometry Methods and Protocols
Edited by
Charlotte H. Clarke Department of Translational Research, The University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA
Diane L. Bankert McCarthy Bio-Rad Laboratories, Malvern, PA, USA
Editors Charlotte H. Clarke Department of Translational Research The University of Texas M.D. Anderson Cancer Center Houston 77090, TX, USA [email protected]
Diane L. Bankert McCarthy Bio-Rad Laboratories Malvern, PA, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-417-9 e-ISBN 978-1-61779-418-6 DOI 10.1007/978-1-61779-418-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011941205 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface This book provides an overview of the current applications of SELDI-TOF-MS (surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry), with an emphasis on study and experimental design, data analysis and interpretation, and assay development. SELDI is distinct from other time-of-flight mass spectrometer (TOF-MS) technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment, and sample cleanup on an array surface. For the analyses of crude biological samples, all mass spectrometric techniques must eliminate substances found in the sample (i.e., buffer salts, detergents) which will interfere with MS detection and use fractionation or enrichment techniques to simplify the complex mixture. SELDI significantly reduces the need for off-line sample preparation by derivatized array surfaces to specifically capture and enrich proteins with specific biochemical properties. Washing of the arrays removes nonspecifically bound proteins and interfering compounds, greatly reducing signal suppression in the mass spectrometer caused by salts and detergents. For complex sample types, such as serum or plasma, SELDI analysis is often combined with upstream fractionation and en
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