Synthesis and comparative study on the antimicrobial activity of hybrid materials based on silver nanoparticles (AgNps)
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ORIGINAL ARTICLE
Synthesis and comparative study on the antimicrobial activity of hybrid materials based on silver nanoparticles (AgNps) stabilized by polyvinylpyrrolidone (PVP) Rayna Bryaskova & Daniela Pencheva & Stanislav Nikolov & Todor Kantardjiev
Received: 22 March 2011 / Accepted: 14 July 2011 / Published online: 27 July 2011 # Springer-Verlag 2011
Abstract Hybrid materials based on polyvinylpyrrolidone (PVP) with silver nanoparticles (AgNps) were synthesized applying two different strategies based on thermal or chemical reduction of silver ions to silver nanoparticles using PVP as a stabilizer. The formation of spherical silver nanoparticles with diameter ranging from 9 to 16 nm was confirmed by TEM analysis. UV-vis and FTIR spectroscopy were also applied to confirm the successful formation of AgNps. The antibacterial activity of the synthesized AgNPs/PVP against etalon strains of three different groups of bacteria—Staphylococcus aureus (S. aureus; grampositive bacteria), Escherichia coli (E. coli; gram-negative bacteria), Pseudomonas aeruginosa (P. aeruginosa; nonferment gram-negative bacteria), as well as against spores of Bacillus subtilis (B. subtilis) was studied. AgNps/PVP were tested for the presence of fungicidal activity against different yeasts and mold such as Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, and Aspergillus brasiliensis. The hybrid materials showed
R. Bryaskova (*) : S. Nikolov Department of Polymer Engineering, University of Chemical Technology and Metallurgy, 8 Kl.Ohridski, 1756 Sofia, Bulgaria e-mail: [email protected] D. Pencheva Bul Bio-National Centre of Infectious and Parasitic Diseases, 26 Yanko Sakazov, 1504 Sofia, Bulgaria T. Kantardjiev National Centre of Infectious and Parasitic Diseases, 26 Yanko Sakazov, 1504 Sofia, Bulgaria
a strong antimicrobial effect against the tested bacterial and fungal strains and therefore have potential applications in biotechnology and biomedical science. Keywords Silver nanoparticles, polyvinylpyrrolidone . Antimicrobial activity . Fungi, spores
Introduction Silver in its different chemical forms has been known for a long time as an effective antimicrobial agent with high activity against bacteria, viruses, and fungi [1, 2]. Among them, silver nanoparticles (AgNps) are extensively studied in the recent years because of their possible usage as a strong bactericidal material with an application in biomedical field. Their pronounced antibacterial activity can be attributed to the well-developed surface and large surface area to volume ratio providing better contact with the microorganisms [3] and to the strong cytotoxicity to bacterial cells as a result of their interaction with the functional groups on the bacterial cell surface [4–6]. The most common method for synthesis of stable AgNps is the chemical reduction of silver ions in different stabilizers such as polymers and surfactants [7–11]. Among them, polyvinylpyrrolidone (PVP) attracts considerable attention because of its excellent chemical and physical pr
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