The Effect of Probiotics Supplementation on Gut Microbiota After Helicobacter pylori Eradication: A Multicenter Randomiz
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ORIGINAL RESEARCH
The Effect of Probiotics Supplementation on Gut Microbiota After Helicobacter pylori Eradication: A Multicenter Randomized Controlled Trial Bo Tang . Li Tang . Cheng Huang . Chuan Tian . Ling Chen . Zhijun He . Guodong Yang . Luo Zuo . Guoce Zhao . En Liu . Sumin Wang . Hui Lin . Jialin He . Shiming Yang
Received: September 24, 2020 / Accepted: November 11, 2020 Ó The Author(s) 2020
ABSTRACT Introduction: Helicobacter pylori eradication therapy may lead to the perturbation of gut microbiota. We aim to investigate the impact of probiotics on eradication rate and gut microbiota during eradication therapy. Methods: A total of 162 patients receiving bismuth quadruple therapy were enrolled and randomly assigned to groups given probiotics (n = 83) or placebo (n = 79) for 4 weeks. Fecal Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40121020-00372-9) contains supplementary material, which is available to authorized users. B. Tang L. Tang G. Zhao E. Liu S. Wang H. Lin J. He (&) S. Yang (&) Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China e-mail: [email protected] S. Yang e-mail: [email protected] C. Huang Department of Gastroenterology, Chongqing Iron and Steel General Hospital, Chongqing, China C. Tian Department of Gastroenterology, Nanchuan People’s Hospital, Nanchuan, China
samples were collected before treatment and 2, 4, 6, and 8 weeks after eradication therapy. Gut microbiota was analyzed by 16S rRNA highthroughput sequencing. Results: The eradication rates in the placebo and probiotics group were 82.43% and 87.01%, respectively (P [ 0.05). Compared with baseline, alpha and beta diversity was significantly altered 2 weeks after eradication in both groups, which was restored at week 8. There were no significant differences in diversity between the two groups. H. pylori eradication therapy resulted in enrichment of some detrimental bacteria taxa such as Shigella, Klebsiella, and Streptococcus, while probiotics supplementation could rapidly restore these taxa levels after eradication and L. Chen Department of Gastroenterology, 958 Hospital of PLA, Chongqing, China Z. He Department of Gastroenterology, Chongqing Fifth People’s Hospital, Chongqing, China G. Yang Department of Gastroenterology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China L. Zuo Department of Gastroenterology, Affiliated Hospital of Chengdu Medical College, Chengdu, China
Infect Dis Ther
increase the taxa of Bacillus and Lactobacillales. Functional analysis revealed that lipopolysaccharide biosynthesis and polymyxin resistance pathways were significantly enriched after eradication, while probiotics supplementation mainly enriched the cofactors and vitamins metabolism pathways. Increased relative abundances of Roseburia and Dialister were associated with the positive eradication outcome. Conclusions: Probiotics supplementation might help to construct a beneficial profile of gut microbiota
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