The protective effects of polyamines on salinity stress tolerance in foxtail millet ( Setaria italica L.), an important
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RESEARCH ARTICLE
The protective effects of polyamines on salinity stress tolerance in foxtail millet (Setaria italica L.), an important C4 model crop Periyasamy Rathinapriya1 • Subramani Pandian1 • Kasinathan Rakkammal1 Manoharan Balasangeetha1 • Rajaiah Alexpandi1 • Lakkakula Satish2 • Ramakrishnan Rameshkumar1 • Manikandan Ramesh1
•
Received: 18 March 2020 / Revised: 26 June 2020 / Accepted: 14 August 2020 Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Soil salinity is a major abiotic stress that adversely affects crop growth, development and productivity worldwide. In this study, the individual and synergistic roles of putrescine (Put) and spermidine (Spd) in salinity stress tolerance of foxtail millet (Setaria italica L.) was assessed. In the present study, plants treated with combined biogenic amines Put ? Spd possess very efficient antioxidant enzyme systems which help to control the uninhibited oxidation and protect the plants from oxidative damage by ROS scavenging. Additionally, lower concentration of Put ? Spd under NaCl stress showed reduced hydrogen peroxide, electrolyte leakage and caspase-like activity than control. FTIR analysis underlying the ability of PAs induced tolerance and the chemical bonds of Put ? Spd treated plants were reminiscent of control
plants. Moreover, histochemical analysis with 20 ,70 – dichlorofluorescein diacetate (DCF-DA), 3,30 –Diaminobenzidine (DAB) and nitrotetrazolium blue chloride (NBT) revealed that ROS accumulation was inhibited by combined PAs under salt stress condition. These results showed that Put ? Spd significantly improve the endogenous PAs, which enhance high-salinity stress tolerance by detoxifying ROS. For the first time, the synergistic ROS scavenging ability of Put along with Spd was investigated upon salinity tolerance in C4 model foxtail millet crop. Overall, our findings illustrated the implication for improving salinity tolerance of agronomically important crop species.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00869-0) contains supplementary material, which is available to authorized users. & Manikandan Ramesh [email protected] 1
Department of Biotechnology, Science Campus, Alagappa University, KaraikudiTamil Nadu, 630 003, India
2
Department of Biotechnology Engineering, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of Negev, 84105 Beer Sheva, Israel
123
Physiol Mol Biol Plants
Graphic abstract
Keywords Confocal laser scanning microscope Fourier transform-infrared spectroscopy Histochemical analysis Polyamines Reactive oxygen species Salinity stress Abbreviations APX Ascorbate peroxidae CAT Catalase CLSM Confocal laser scanning microscope DAB 3,3-diaminobenzidine DAO Diamine oxidase EL Electrolyte leakage FTIR Fourier transform-infrared spectroscopy GPX Guaiacol peroxidise GR Glutathione reductase GSH Glutathione GSSG Glutathione disulfide H2O2 Hydrogen peroxide MDA Malondialdehyde NaCl Sodium chloride NADH
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