Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biologi
- PDF / 731,164 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 30 Downloads / 154 Views
RESEARCH PAPER
Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples Andreas Gruber 1 & Riccarda Müller 1 & Alessa Wagner 2 & Silvia Colucci 3,4 & Maja Vujić Spasić 2 & Kerstin Leopold 1 Received: 31 January 2020 / Revised: 17 March 2020 / Accepted: 23 March 2020 # The Author(s) 2020
Abstract Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are time-consuming, and require special sample preparation. Therefore, the development of straightforward and rapid analytical methods for enhanced, multi-trace element determination in biological samples is an important and raising field of trace element analysis. Herein, we report on the development and validation of a reliable method based on total reflection X-ray fluorescence (TXRF) analysis to precisely quantify iron and other trace metals in a variety of biological samples, such as the liver, parenchymal and non-parenchymal liver cells, and bone marrow–derived macrophages. We show that TXRF allows fast and simple one-point calibration by addition of an internal standard and has the potential of multi-element analysis in minute sample amounts. The method was validated for iron by recovery experiments in homogenates in a wide concentration range from 1 to 1600 μg/L applying well-established graphite furnace atomic absorption spectrometry (GFAAS) as a reference method. The recovery rate of 99.93 ± 0.14% reveals the absence of systematic errors. Furthermore, the standard reference material “bovine liver” (SRM 1577c, NIST) was investigated in order to validate the method for further biometals. Quantitative recoveries (92–106%) of copper, iron, zinc, and manganese prove the suitability of the developed method. The limits of detection for the minute sample amounts are in the low picogram range.
Keywords Total reflection X-ray fluorescence spectrometry . Iron trace analysis . Biometal trace analysis . Bone marrow–derived macrophages . Liver cells . Liver tissues
Introduction Published in the topical collection featuring Female Role Models in Analytical Chemistry. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02614-8) contains supplementary material, which is available to authorized users. * Kerstin Leopold [email protected] 1
Institute of Analytical and Bioanalytical Chemistry, Ulm University, 89081 Ulm, Germany
2
Institute of Comparative Molecular Endocrinology, Ulm University, 89081 Ulm, Germany
3
Department of Pediatric Hematology, Oncology and Immunology, University of Heidelberg, 69120 Heidelberg, Germany
4
Molecular Medicine Partnership Unit, 69120 Heidelberg, Germany
Essential trace elements, such as iron, zinc, copper, manganese, and selenium, are required to maintain physio
Data Loading...
