Transcription Factors Methods and Protocols
In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field desc
- PDF / 8,630,167 Bytes
- 410 Pages / 504 x 720 pts Page_size
- 68 Downloads / 213 Views
in
Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
Transcription Factors Methods and Protocols
Edited by
Paul J. Higgins Center for Cell Biology and Cancer Research, Albany Medical College, Albany, New York, USA
Editor Paul J. Higgins, Ph.D. Center for Cell Biology and Cancer Research Albany Medical College Albany New York USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-737-2 e-ISBN 978-1-60761-738-9 DOI 10.1007/978-1-60761-738-9 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010929390 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is a part of Springer Science+Business Media (www.springer.com)
Preface In compiling “Transcription Factors: Methods and Protocols,” it was the intent to present a methodological resource that highlights several of the most exciting recent developments that have expanded our appreciation for the complexity of transcriptional controls in mammalian cells. This volume is divided into four major sections and includes chapters that specifically focus on the newest experimental approaches that investigators can utilize to: (1) probe mechanisms underlying transcription factor nuclear-cytoplasmic trafficking (i.e., “shuttling”) and (2) assess the impact of post-translational modifications on transcription factor function including regulation of the coupled ubiquitination/degradation process. In each instance, the protocols highlight specific transcription factor family members with particular relevance to human disease. The aim of this book is to present a compilation of state-of-the-art techniques and concepts important to elucidating controls on transcription factor intracellular localization and activity. The topics selected, therefore, a
Data Loading...
