Wood forming tissue-specific expression of PdSuSy and HCHL increases holocellulose content and improves saccharification
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ORIGINAL PAPER
Wood forming tissue‑specific expression of PdSuSy and HCHL increases holocellulose content and improves saccharification in Populus Yang Zhang1,2,3 · Hua Xu2,3 · Yingzhen Kong4 · Jiawen Hua2,3,5 · Xianfeng Tang2,3 · Yamei Zhuang1,2,3 · Yue Bai5 · Gongke Zhou2,3 · Guohua Chai2,3,6
Received: 10 December 2019 / Accepted: 14 January 2020 © The Author(s) 2020
Abstract Development of strategies to deconstruct lignocellulosic biomass in tree species is essential for biofuels and biomaterials production. We applied a wood forming tissue-specific system in a hybrid poplar to express both PdSuSy (a sucrose synthase gene from Populus deltoides × P. euramericana that has not been functionally characterized) and HCHL (the hydroxycinnamoyl-CoA hydratase-lyase gene from Pseudomonas fluorescens, which inhibits lignin polymerization in Arabidopsis). The PdSuSy-HCHL overexpression poplars correspondingly driven by the promoters Project funding: This work was funded by National Key Program on Transgenic Research (2018ZX08020002), National Key Scientific Research Project of China (2016YFD0600104), National Natural Science Foundation of China (31670606, 31570670, 31770315 and 31701068), Major Basic Research Project of Shandong Natural Science Foundation (ZR2018ZC0335), Shandong Provincial Natural Science Foundation (ZR2017BC096 and ZR2017BC078) and Taishan Scholar Program of Shandong (to G.Z.).
of Arabidopsis AtCesA7 and AtC4H resulted in a significant increase in cellulose (> 8%), xylan (> 12%) and glucose (> 29%) content, accompanying a reduction in galacturonic acid (> 36%) content, compared to control plants. The saccharification efficiency of these overexpression poplars was dramatically increased by up to 27%, but total lignin content was unaffected. These transgenic poplars showed inhibited growth characteristics, including > 16% reduced plant height, > 10% reduced number of internodes, and > 18% reduced fresh weight after growth of 4 months, possibly due to relatively low expression of HCHL in secondary xylem. Our results demonstrate the structural complexity and interaction of the cell wall polymers in wood tissue and outline a potential method to increase biomass saccharification in woody species. Keywords Saccharification · Biomass · Cell wall composition · Growth · Poplar
The online version is available at http://www.springerlink.com. Corresponding editor: Yanbo Hu. * Gongke Zhou [email protected]
4
College of Agronomy, Qingdao Agricultural University, Qingdao 266109, People’s Republic of China
* Guohua Chai [email protected]
5
Forestry College, Inner Mongolia Agricultural University, Huhhot 010018, People’s Republic of China
1
University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China
6
College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, People’s Republic of China
2
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People’s Republic of
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