Yeast Functional Genomics and Proteomics Methods and Protocols
Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene function
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Yeast Functional Genomics and Proteomics Methods and Protocols
Edited by
Igor Stagljar Department of Biochemistry & Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada
Editor Igor Stagljar, Ph.D. Department of Biochemistry & Department of Molecular Genetics Terrence Donnelly Centre for Cellular and Biomolecular Research University of Toronto Toronto, ON Canada
ISSN: 1064-3745 e-ISSN: 1940-6029 ISBN: 978-1-934115-71-8 e-ISBN: 978-1-59745-540-4 DOI: 10.1007/978-1-59745-540-4 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2008944036 © Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: Taken from Chapter 5, Figure 6B. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Dedication This book is dedicated to my parents Sonja and Mirko Stagljar for their great guidance and support in my life
Preface Often defined as “the unicellular human” and “everybody’s favorite fungus,” the baker’s yeast Saccharomyces cerevisiae has long been considered one of the most highly studied model organisms in the study of basic cellular processes. Along with this notion, yeastbased functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm, and human gene functions. More than 1,000 different papers and hundreds of reviews dealing with functional genomics and proteomics in yeast have appeared, but no comprehensive yeastbased functional genomics and proteomics textbook has yet been written and published. This book aims to be the standard tex
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