Rho GTPases Methods and Protocols
This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, bioch
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Francisco Rivero Editor
Rho GTPases Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Rho GTPases Methods and Protocols Second Edition
Edited by
Francisco Rivero The Hull York Medical School, University of Hull, Hull, United Kingdom
Editor Francisco Rivero The Hull York Medical School University of Hull Hull, United Kingdom
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8611-8 ISBN 978-1-4939-8612-5 (eBook) https://doi.org/10.1007/978-1-4939-8612-5 Library of Congress Control Number: 2018947826 © Springer Science+Business Media, LLC, part of Springer Nature 2018 Chapter 13 is licensed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/). For further details see license information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Rho GTPases entered the cell biology field in the 1990s, when their roles in reorganizing the actin cytoskeleton were first demonstrated in yeast and mammalian cells. This was followed soon by the identification and characterization of families of effectors and regulators. Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been assigned central roles in a long list of key cellular processes that include establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activat
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