Schizosaccharomyces pombe Methods and Protocols
This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to thei
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Teresa L. Singleton Editor
Schizosaccharomyces pombe Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Schizosaccharomyces pombe Methods and Protocols
Edited by
Teresa L. Singleton Department of Biological Sciences, Winston-Salem State University, Winston-Salem, NC, USA
Editor Teresa L. Singleton Department of Biological Sciences Winston-Salem State University Winston-Salem, NC, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7545-7 ISBN 978-1-4939-7546-4 (eBook) https://doi.org/10.1007/978-1-4939-7546-4 Library of Congress Control Number: 2017963311 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication I dedicate my first book editing experience to God, my lovely daughter Shakyrah, and all undergraduate student researchers.
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Preface The unicellular organism has facilitated our understanding of cellular mechanisms in higher eukaryotes for years. The prokaryote, Escherichia coli, has served as a model organism in the genetic engineering laboratory due to the inexpensive means to grow it and its fast generation time. E. coli serves as an excellent host for the production of proteins, bacteriophage infection, and the use of conjugation to study antibiotic resistance. The rise of more genetic diseases in higher eukaryotes has forced the research community to adopt yet another model organism. Schizosaccharomyces pombe (fission yeas
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