Sequential fed-batch fermentation of 1,3-propanediol from glycerol by Clostridium butyricum DL07
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BIOTECHNOLOGICAL PRODUCTS AND PROCESS ENGINEERING
Sequential fed-batch fermentation of 1,3-propanediol from glycerol by Clostridium butyricum DL07 Xiao-Li Wang 1 & Jin-Jie Zhou 1 & Jun-Tao Shen 1 & Ya-Feng Zheng 1 & Ya-qin Sun 1 & Zhi-Long Xiu 1 Received: 17 May 2020 / Revised: 30 August 2020 / Accepted: 23 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The demand for 1,3-propanediol (1,3-PDO) has increased sharply due to its role as a monomer for the synthesis of polytrimethylene terephthalate (PTT). Although Clostridium butyricum is considered to be one of the most promising bioproducers for 1,3-PDO, its low productivity hinders its application on industrial scale because of the longer time needed for anaerobic cultivation. In this study, an excellent C. butyricum (DL07) strain was obtained with high-level titer and productivity of 1,3-PDO, i.e., 104.8 g/L and 3.38 g/(L•h) vs. 94.2 g/L and 3.04 g/(L•h) using pure or crude glycerol as substrate in fedbatch fermentation, respectively. Furthermore, a novel sequential fed-batch fermentation was investigated, in which the next bioreactor was inoculated by C. butyricum DL07 cells growing at exponential phase in the prior bioreactor. It could run steadily for at least eight cycles. The average concentration of 1,3-PDO in eight cycles was 85 g/L with the average productivity of 3.1 g/ (L•h). The sequential fed-batch fermentation could achieve semi-continuous production of 1,3-PDO with higher productivity than repeated fed-batch fermentation and would greatly contribute to the industrial production of 1,3-PDO by C. butyricum. Key points • A novel C. butyricum strain was screened to produce 104.8 g/L 1,3-PDO from glycerol. • Corn steep liquor powder was used as a cheap nitrogen source for 1,3-PDO production. • A sequential fed-batch fermentation process was established for 1,3-PDO production. • An automatic glycerol feeding strategy was applied in the production of 1,3-PDO. Keywords 1,3-Propanediol . Glycerol . Clostridium butyricum . Sequential fed-batch fermentation
Introduction 1,3-Propanediol is a versatile chemical compound with wide application in plastics, lubricants, cosmetics, pharmaceuticals, and other fields. Especially, it is a crucial monomer for the synthesis of a novel biodegradable polymer, polytrimethylene terephthalate (PTT). PTT has been used in the textile industry for making carpets, apparel, and fiber owing to its superior properties such as easy dye ability, good resilience, and softness (Liu et al. 2010; Kaur et al. 2012a; Lee et al. 2015). The demand of 1,3-PDO is growing rapidly with the profitable textile industry. At present, the production of 1,3-PDO can * Zhi-Long Xiu [email protected] 1
School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning Province, People’s Republic of China
follow chemical and biological routes. Considering the mild reaction conditions and sustainable development of bioproduction, the bioconversion of 1,3-PDO has attracted great interest from researche
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