Serendipita restingae sp. nov. (Sebacinales): an orchid mycorrhizal agaricomycete with wide host range
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ORIGINAL ARTICLE
Serendipita restingae sp. nov. (Sebacinales): an orchid mycorrhizal agaricomycete with wide host range Yohan Fritsche1 · Morgana E. Lopes1 · Marc‑André Selosse3,4 · Valdir M. Stefenon1 · Miguel P. Guerra1,2 Received: 6 June 2020 / Accepted: 21 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The Serendipitaceae family was erected in 2016 to accommodate the Sebacinales ‘group B’ clade, which contains peculiar species of cultivable root–associated fungi involved in symbiotic associations with a wide range of plant species. Here we report the isolation of a new Serendipita species which was obtained from protocorms of the terrestrial orchid Epidendrum fulgens cultivated in a greenhouse. This species is described based on phylogenetic analysis and on its microscopic and ultrastructural features in pure culture and in association with the host’s protocorms. Its genome size was estimated using flow cytometry, and its capacity to promote the germination of E. fulgens seeds and to associate with roots of Arabidopsis thaliana was also investigated. Serendipita restingae sp. nov. is closely related to Serendipita sp. MAFF305841, isolated from Microtis rara (Orchidaceae), from which it differs by 14.2% in the ITS region and by 6.5% in the LSU region. It produces microsclerotia formed of non-monilioid hyphae, a feature that was not reported for the Sebacinales hitherto. Serendipita restingae promoted the germination of E. fulgens seeds, forming typical mycorrhizal pelotons within protocorm cells. It was also able to colonize the roots of Arabidopsis thaliana under in vitro conditions. Arabidopsis plants grown in association with S. restingae increased their biomass more than fourfold. Serendipita restingae is the first Serendipitaceae species described for the Americas. Keywords Serendipitaceae · Symbiotic · Endophyte · Epidendrum · New species · Microsclerotia
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00572-020-01000-7) contains supplementary material, which is available to authorized users. * Miguel P. Guerra [email protected] 1
Plant Developmental Physiology and Genetics Laboratory, Department of Plant Science, Federal University of Santa Catarina, SC 88034‑001 Florianópolis, Brazil
2
Graduate Program in Agricultural and Natural Ecosystems, Federal University of Santa Catarina, Curitibanos, SC 89520‑000, Brazil
3
Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National D’Histoire Naturelle, CNRS, Sorbonne Université, EPHE, 39, 57 rue Cuvier, F‑750055 Paris, CP, France
4
Faculty of Biology, University of Gdańsk, ul. Wita Stwosza 59, 80‑308 Gdansk, Poland
The Sebacinales are a monophyletic order of Basidiomycetes involved in a broad range of symbiotic associations with the roots of land plants (Weiß et al. 2004, 2016; Oberwinkler et al. 2013). They likely originated 300–400 million years ago (He et al. 2019) and are globally distributed (Garnica et al. 2016),
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