Sphingomonas palmae sp. nov. and Sphingomonas gellani sp. nov., endophytically associated phyllosphere bacteria isolated
- PDF / 567,510 Bytes
- 16 Pages / 547.087 x 737.008 pts Page_size
- 67 Downloads / 223 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL PAPER
Sphingomonas palmae sp. nov. and Sphingomonas gellani sp. nov., endophytically associated phyllosphere bacteria isolated from economically important crop plants Munusamy Madhaiyan . Venkatakrishnan Sivaraj Saravanan . Joseph S. Wirth . Tan Hian Hwee Alex . Soo-Jin Kim . Hang-Yeon Weon . Soon-Wo Kwon . William B. Whitman . Lianghui Ji
Received: 27 March 2020 / Accepted: 31 August 2020 Ó Springer Nature Switzerland AG 2020
Abstract In this study, two endophytic bacterial strains designated JS21-1T and S6-262T isolated from leaves of Elaeis guineensis and stem tissues of Jatropha curcas respectively, were subjected for polyphasic taxonomic approach. On R2A medium, colonies of strains JS21-1T and S6-262T are orange and yellow, respectively. Phylogenetic analyses using 16S rRNA gene sequencing and whole-genome sequences placed the strains in distinct clades but within the genus Sphingomonas. The DNA G ? C content of JS21-1T and S6-262T were 67.31 and 66.95%, respectively. Furthermore, the average nucleotide identity and digital DNA-DNA The 16S rRNA gene sequences of the strains JS21-1T and S6262T were deposited in GenBank/EMBL/DDBJ under the accession numbers are KX893410 and JQ660211, respectively. The draft genome sequences of the strains JS21-1T and S6262T are available at the following accessions FNZZ00000000.1 and FOCF00000000.1, respectively.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01468-5) contains supplementary material, which is available to authorized users. M. Madhaiyan (&) T. H. H. Alex L. Ji (&) Biomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore e-mail: [email protected] L. Ji e-mail: [email protected]
hybridization values of strains JS21-1T and S6-262T with phylogenetically related Sphingomonas species were lower than 95% and 70% respectively. The chemotaxonomic studies indicated that the major cellular fatty acids of the strain JS21-1T were summed feature 8 (C18:1 x7c and/or C18:1 x6c), C16:0, and C14:0 2OH; strain S6-262T possessed summed feature 3 (C16:1 x7c and/or iso-C15:0 2-OH) and summed feature 8 (C18:1 x6c and/or C18:1 x7c). The major quinone was Q10, and the unique polyamine observed was homospermidine. The polar lipid profile comprised of mixture of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and certain uncharacterised phospholipids and lipids. Based on this polyphasic evidence, strains JS21-1T and S6-262T represent two novel species of the genus Sphingomonas, for which the names Sphingomonas palmae sp. nov. and Sphingomonas gellani sp. nov. are proposed, respectively. The type strain of Sphingomonas palmae sp. nov. is JS21-1T (= DSM 27348T = KACC 17591T) and the type strain of Sphingomonas gellani sp. nov. is S6262T (= DSM 27346T = KACC 17594T). V. S. Saravanan Department of Microbiology, Indira Gandhi College of A
Data Loading...
