Rhodocytophaga rosea sp. nov. and Nibribacter ruber sp. nov., two radiation-resistant bacteria isolated from soil
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ORIGINAL PAPER
Rhodocytophaga rosea sp. nov. and Nibribacter ruber sp. nov., two radiation-resistant bacteria isolated from soil Yuna Park . Soohyun Maeng . Joo Hyun Han . Sang Eun Lee . Jing Zhang . Min-Kyu Kim . In-Tae Cha . Ki-eun Lee . Byoung-Hee Lee . Myung Kyum Kim
Received: 6 April 2020 / Accepted: 19 October 2020 Ó Springer Nature Switzerland AG 2020
Abstract Two bacterial strains, 172606-1T and BT10T, were isolated from soil, Korea. Both strains were Gram-stain-negative and rod-shaped bacteria. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 172606-1T formed a distinct lineage within the family Cytophagaceae (order Cytophagales, class Cytophagia, phylum Bacteroidetes). Strain 172606-1T was most closely related to a member of the genus Rhodocytophaga (93.8% 16S rRNA gene sequence similarity to Rhodocytophaga aerolata 5416T-29T). The complete genome sequence of strain 172606-1T is 8,983,451 bp size. Optimal growth occurred at 25 °C and pH 7.0 without NaCl. The major cellular fatty acids were identified as
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01488-1) contains supplementary material, which is available to authorized users. Y. Park S. Maeng J. H. Han S. E. Lee M. K. Kim (&) Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 01797, Republic of Korea e-mail: [email protected] J. Zhang M.-K. Kim Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea
iso-C15:0 and C16:1 x5c. The major respiratory quinone was MK-7. The major polar lipid was phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BT10T belongs to the genus Nibribacter and is closely related to Nibribacter koreensis GSR 3061T (96.5%), Rufibacter glacialis MDT1-10-3T (95.7%), Rufibacter sediminis H-1T (95.1%) and Rufibacter quisquiliarum CAI-18bT (94.9%). The complete genome sequence of strain BT10T is 4,374,810 bp size. The predominant ([ 10%) cellular fatty acids of strain BT10T were iso-C15:0 and summed feature 4 (anteiso-C17:1 B/iso-C17:1 I) and a predominant quinone was MK-7. In addition, strain BT10T has phosphatidylethanolamine (PE) as the major polar lipid. On the basis of biochemical, chemotaxonomic and phylogenetic analyses, strain 172606-1T represents a novel bacterial species of the genus Rhodocytophaga, for which the name Rhodocytophaga rosea is proposed and strain BT10T represents a novel species of the genus Nibribacter, for which the name Nibribacter ruber is proposed. The type strains of Rhodocytophaga rosea and Nibribacter ruber are 172606-1T (= KCTC 62096T = NBRC 114410T) and BT10T (= KCTC 62607T = NBRC 114383T), respectively. Keywords Cytophagales Novel species Radiation-resistant Taxonomy
I.-T. Cha K. Lee B.-H. Lee National Institute of Biological Resources, Hwangyeongro 42, Seo-gu, Incheon 22689, Republic of Korea
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