Pontibacter fetidus sp. nov. and Pontibacter burrus sp. nov., isolated from the soil
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ORIGINAL PAPER
Pontibacter fetidus sp. nov. and Pontibacter burrus sp. nov., isolated from the soil Yuna Park1 · Soohyun Maeng1 · Joo Hyun Han1 · Sang Eun Lee1 · Yoonjee Chang2 · Myung Kyum Kim1 Received: 1 June 2020 / Revised: 11 September 2020 / Accepted: 1 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Two novel strains, BT213T and BT327T, were isolated from the soil collected in Uijeongbu city, Korea. Cells of strains were Gram negative, aerobic, and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains BT213T and BT327T formed two distinct lineages within the family Hymenobacteraceae (order Cytophagales, class Cytophagia). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BT213T and BT327T belonged to the genus Pontibacter. Strain BT213T showed the highest similarities of 97.8% with Pontibacter deserti JC215T. Strain BT327T showed the highest sequence similarity of 96.5% with Pontibacter mucosus PB3T. Optimal growth occurred at 25 °C, pH 7, and in the absence of NaCl. The major cellular fatty acid of strains B T213T and B T327T were iso-C15:0 and T T summed feature 4 (iso-C17:1 I/anteiso-C17:1 B). Strains B T213 and BT327 had MK-7 as major respiratory quinone and phosphatidylethanolamine as major polar lipids. The genome size of strains B T213T and BT327T were 4,072,018 bp and T 4,314,171 bp, respectively. The genomic G + C mol% of strains BT213 and BT327T are 45.6% and 46.1%, respectively. Based on biochemical, chemotaxonomic, and phylogenetic analysis, two novel species Pontibacter fetidus BT213T (KCTC 72345T = NBRC 114379T) and Pontibacter burrus BT327T (KCTC 7 2412T = NBRC 114376T) are proposed as type strains. Keywords Novel species · Pontibacter · Pontibacter fetidus · Pontibacter burrus · Taxonomy
Introduction The genus Pontibacter is a member of the family Hymenobacteraceae in the phylum Bacteroidetes. Pontibacter was first proposed by Nedashkovskaya et al. (2005) and amended by Wang et al. (2010). The genus Pontibacter contains 32 species (https://www.bacter io.net/pontibacter). The type Communicated by Erko stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02070-z) contains supplementary material, which is available to authorized users. * Yoonjee Chang [email protected] * Myung Kyum Kim [email protected] 1
Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul 139– 774, Republic of Korea
Department of Food and Nutrition, Kookmin University, Seoul 02707, Republic of Korea
2
species of Pontibacter is P. actiniarum (Nedashkovskaya et al. 2005). Species in the genus Pontibacter have been isolated from various places such as a desert (Subhash et al. 2014; Xu et al. 2012; Zhou et al. 2007), deep-sea sediment (Wu et al. 2016), pond sediment (Nayyar et al. 2016), and forest (Xu et al. 2012). In the course of screening novel bacterial species
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