Silver nanoparticles against acute hepatopancreatic necrosis disease (AHPND) in shrimp and their depuration kinetics
- PDF / 2,207,420 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 67 Downloads / 160 Views
23RD INTERNATIONAL SEAWEED SYMPOSIUM, JEJU
Silver nanoparticles against acute hepatopancreatic necrosis disease (AHPND) in shrimp and their depuration kinetics Maribel Maldonado-Muñiz 1 & Carlos Luna 2 & Raquel Mendoza-Reséndez 2 & Enrique Díaz Barriga-Castro 3 Sonia Soto-Rodriguez 4 & Denis Ricque-Marie 1 & Lucia Elizabeth Cruz-Suarez 1
&
Received: 9 July 2019 / Revised and accepted: 9 October 2019 # Springer Nature B.V. 2019
Abstract Ag-based nanoparticles (NPs) were successfully produced through green synthesis using an aqueous extract from the cultivated seaweed Ulva clathrata as the reducing and stabilizing agent. The biosynthesized NPs had spherical to polymorphic shapes with an average size of 9.5 nm. Microstructural and compositional studies revealed that these particles contained face-centred cubic crystallites of metallic Ag and AgCl. Characteristic peaks in the Fourier-transform infrared spectrum (FTIR) and Raman studies revealed the presence of functional bioactive metabolites from the seaweed extract, such as proteins, polysaccharides, and polyphenols, which are responsible for forming and stabilizing Ag/AgCl NPs. The biosynthesized Ag/AgCl NPs exhibited an important in vitro antibacterial effect against three Vibrio parahaemolyticus strains isolated from farmed shrimp affected with acute hepatopancreatic necrosis disease (AHPND) in northwestern Mexico. Litopenaeus vannamei shrimp were exposed for 7 days to feeds supplemented with Ag/AgCl NPs at 10, 100, 1000, or 10,000 ppm (Ag nominal dietary concentrations). Dietary NP supplement did not affect shrimp survival, growth, or feed conversion ratio, but high concentrations (1000 and 10,000 ppm) decreased the hepatosomatic index significantly. The short-term consumption of Ag/AgCl NPs produced a significant dosedependent bioaccumulation of Ag in the hepatopancreas and to a lesser extent in the cuticle, while bioaccumulation in the muscle was not significant. The depuration study confirmed a fast Ag assimilation in shrimp’s hepatopancreas and showed a fast depuration rate in the hepatopancreas as well. Keywords Antibacterial activity . Green seaweed . Green synthesis . Litopenaeus vannamei . Vibrio parahaemolyticus
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10811-019-01948-w) contains supplementary material, which is available to authorized users. * Lucia Elizabeth Cruz-Suarez [email protected] 1
Facultad de Ciencias Biologicas (FCB), Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N, 66455 San Nicolás de los Garza, Nuevo Leon, Mexico
2
Facultad de Ciencias Fisico Matematicas (FCFM), Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N, 66455 San Nicolas de los Garza, Nuevo Leon, Mexico
3
Centro de Investigacion en Quimica Aplicada (CIQA), Blvd. Enrique Reyna Hermosillo, No. 140, 25294 Saltillo, Coahuila, Mexico
4
Centro de Investigación Alimentacion y Desarrollo, Av. Sabalo-Cerritos s/n, Estero del Yugo, 82000 Mazatlan, Sinaloa, Mexico
Shrimp farming
Data Loading...
