Bacterial-mediated synthesis of silver nanoparticles and their significant effect against pathogens
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ENVIRONMENTAL TOXICOLOGY AND BIOGEOCHEMISTRY OF ECOSYSTEMS
Bacterial-mediated synthesis of silver nanoparticles and their significant effect against pathogens Saira Saeed 1 & Atia Iqbal 1 & Muhammad Aqeel Ashraf 2 Received: 28 November 2019 / Accepted: 1 January 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Silver nanoparticles are potent antimicrobials and could be used as a promising alternative of conventional antibiotics. The aim of this study was to isolate bacteria from soil that have ability to produce AgNPs by secondary metabolite activity and their elucidation against human pathogens. These strains Escherichia coli, Exiguobacterium aurantiacumm, and Brevundimonas diminuta with NCBI accession number MF754138, MF754139, and MF754140 respectively were grown for secondary metabolite production. The nanoparticles were confirmed and characterized by UV-Vis spectroscopy and transmission electron microscopy. The optimization study was also carried out to obtain the maximum production of silver nanoparticles. Three parameters, temperature, pH, and AgNO3 concentration, were used to optimize the production of silver nanoparticles. Antimicrobial potential of these nanoparticles was addressed on the Muller-Hinton Agar, and their zones of inhibitions were measured. TEM analysis revealed the size and shape of the silver nanoparticles. All types of AgNPs were spherical in shape; their size range is from 5 to 50 nm. The findings of optimization study showed the maximum production of silver nanoparticles at the pH 9, temperature 37 °C, and 1 mM AgNO3 concentration. All the strains exhibited the great potential as antimicrobial agents against MRSA and several other MDR bacteria with minimum 10 mm to maximum 28 mm zone of inhibition. It was concluded that the present study is an eco-friendly approach for the synthesis of AgNPs that will be beneficial to control the nosocomial infections triggered by MRSA and other human pathogens. Keywords Antimicrobial activity . Methicillin-resistant Staphylococcus aureus (MRSA) . Silver nanoparticles (AgNPs) . Transmission electron microscopy (TEM) . UV-Vis spectroscopy
Introduction Nanotechnology is an emergent field which is influencing all the areas of life, and soon it will be the next industrial revolution. This technology introduces the innovation in the field of manufacturing, medicine industry, biotechnology, nano-electronics, and energy. Several physical, chemical, and biological methods are used for the synthesis of nanoparticles, but Responsible Editor: Philippe Garrigues * Saira Saeed [email protected] * Atia Iqbal [email protected] 1
Department of Microbiology and Molecular Genetics, The Women University, Multan, Pakistan
2
School of Environmental Studies, China University of Geosciences, Wuhan, China
biological approach is more reliable, because biological synthesis is ecofriendly and cost effective and required less time for the production (Gandhi and Khan 2016). Nanoparticles are the particles of nano sized and occupying the exce
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