Site-Specific Protein Labeling Methods and Protocols
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters
- PDF / 9,471,172 Bytes
- 273 Pages / 504.63 x 737.01 pts Page_size
- 38 Downloads / 191 Views
Arnaud Gautier Marlon J. Hinner Editors
Site-Specific Protein Labeling Methods and Protocols
METHODS
IN
M O L E C U L A R B I O LO G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Site-Specific Protein Labeling Methods and Protocols
Edited by
Arnaud Gautier Department of Chemistry, École Normale Supérieure, Paris, France
Marlon J. Hinner Sensitive Farbstoffe GbR, Munich, Germany
Editors Arnaud Gautier Department of Chemistry École Normale Supérieure Paris, France
Marlon J. Hinner Sensitive Farbstoffe GbR Munich, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-2271-0 ISBN 978-1-4939-2272-7 (eBook) DOI 10.1007/978-1-4939-2272-7 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014958475 © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover Image Description: Confocal and STED microscopy image of methanol fixed U2OS cell expressing microtubule binding fusion protein SNAP-Cep41. SNAP-tagged protein is stained with cell permeable SiR-SNAP substrate before fixation. Cover Courtesy: The image is courtesy of Gražvydas Lukinavicˇius and Kai Johnsson, École Polytechnique Fédérale de Lausanne Printed on acid-free paper Humana Press is a brand of Springer Springer is part of Springer Science+Business Media (www.springer.com)
Preface Recombinant DNA technologies have revolutionized the way biologists study and manipulate proteins. The ability to produce chimeric proteins by inserting a peptide sequence before, after, or within a protein through genetic manipulation has led to the development of a multitude of techniques that render a protein of interest unique merely by adding an encoded label. Prominent examples are the introduction of small epitopes for immunolabeling, the use of affinity tags for protein purification, and the fusion to f
Data Loading...
