Streptomyces castaneus sp. nov., a novel actinomycete isolated from the rhizosphere of Peucedanum praeruptorum Dunn
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ORIGINAL PAPER
Streptomyces castaneus sp. nov., a novel actinomycete isolated from the rhizosphere of Peucedanum praeruptorum Dunn Shuyu Zhou1 · Zhilei Li1 · Lu Bai1 · Kai Yan1 · Junwei Zhao1 · Chang Lu1 · Chongxi Liu1 · Xiangjing Wang1 · Wensheng Xiang1,2
Received: 18 May 2016 / Revised: 23 June 2016 / Accepted: 19 July 2016 © Springer-Verlag Berlin Heidelberg 2016
Abstract During an investigation of microbial diversity in medicinal herbs, a novel actinomycete, strain NEAUQHHV11T was isolated from the rhizosphere of Peucedanum praeruptorum Dunn collected from Xianglu Mountain in Heilongjiang Province, northeast China and characterized using a polyphasic approach. The organism was found to have typical characteristics of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequence also indicated that strain NEAU-QHHV11T belongs to the genus Streptomyces and was most closely related to Streptomyces graminilatus NBRC 108882T (98.7 % sequence similarity) and Streptomyces turgidiscabies NBRC 16080T (98.7 % sequence similarity). The results of DNA–DNA hybridization and some phenotypic characteristics indicated that strain NEAU-QHHV11T could be distinguished from its close phylogenetic relatives.
Communicated by Erko Stackebrandt. Shuyu Zhou and Zhilei Li have contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s00203-016-1274-9) contains supplementary material, which is available to authorized users. * Xiangjing Wang [email protected] * Wensheng Xiang [email protected] 1
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District 150030, Harbin, People’s Republic of China
2
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China
Thus, strain NEAU-QHHV11T represents a novel species of the genus Streptomyces, for which the name Streptomyces castaneus sp. nov. is proposed. The type strain is NEAUQHHV11T (=CGMCC 4.7235T = DSM 100520T). Keywords Streptomyces castaneus sp. nov. · Peucedanum praeruptorum Dunn · Polyphasic taxonomy · 16S rRNA gene
Introduction The genus Streptomyces was first described by Waksman and Henrici (1943) and now consists of more than 700 species with validly published names (http://www.bacterio.net/streptomycesa.html). Members of the genus are aerobic, Gram-positive bacteria which form an extensively branched substrate mycelium with aerial hyphae which differentiate into chains of spores and DNA with high G+C content (Lechevalier and Lechevalier 1970; Kämpfer 2012; Manfio et al. 1995). Indeed, Streptomyces is the largest genus of the phylum Actinobacteria (Kämpfer and Labeda 2006; Lodders and Kämpfer 2007) and constitutes the most predominant component of micro-organisms found in soils, sediments and decaying vegetation (Labeda et al. 2012). Peucedanum pr
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