Crenobacter caeni sp. nov. Isolated from Sludge
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Crenobacter caeni sp. nov. Isolated from Sludge Zhou Yang1 · Jianyi Xu1 · Mengyao Sheng1 · Jiguo Qiu1 · Jianchun Zhu2 · Jun Zhang3 · Jian He1 Received: 18 March 2020 / Accepted: 29 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Strain HX-7-9T was isolated from the activated sludge collected from the outlet of the biochemical treatment facility of agricultural chemical plant in Nanjing, Jiangsu province, PR China. Strain HX-7-9T is Gram staining-negative, rod-shaped, non-spore-forming and flagellated. The 16S rRNA gene-based phylogenetic analysis indicate that strain HX-7-9T belongs to the genus Crenobacter, moderately related to Crenobacter luteus YIM-78141T (94.8% similarity). The genomic DNA G+C content of the strain was 67.5 mol%. Strain HX-7-9T was able to grow at 16–45 °C (optimum at 37 °C), at pH 6.0–8.0 (optimum at pH 7.0) and with 0–1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents were C 16:0 and summed feature 3 ( C16:1ω7c and/or C 16:1ω6c). The respiratory ubiquinone was Q-8. The polar lipid profile is composed of diphosphatidylglycerol (DPG), phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phospholipids (PL), glycolipid (GL) and aminophospholipid (APL). The ANI and dDDH values obtained between the genomes of HX-7-9T and C. luteus YIM-78141T were 79.8 and 19.1%, respectively. On the basis of data from phenotypic, chemotaxonomic and genotypic analysis, strain HX-7-9T represents a novel species of the genus Crenobacter, for which the name Crenobacter caeni sp. nov. is proposed. The type strain is HX-7-9T (= KCTC 72654T = CCTCC AB 2019349T).
Introduction The genus Crenobacter was proposed early by Dong et al. [1]. At the time of writing, the genus contained only 2 species (https://www.bacterio.net/crenobacter.html). The type strains Crenobacter luteus YIM-78141T and Crenobacter cavernae K1W11S-77T were isolated from hot spring and karst cave, respectively [1, 2]. In this study, we report on the polyphasic The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and the whole genome of strains HX-7-9T are MT012982 and JAAGAA000000000, respectively. * Jun Zhang [email protected] * Jian He [email protected] 1
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
2
Laboratory Centre of Life Science, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
3
The College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
study of a Crenobacter-like bacterial strain isolated from the activated sludge of an agricultural chemical plant in Nanjing, China.
Materials and Methods Isolation and Growth Conditions A sludge sample was collected from the outlet of the biochemical treatment facility of agricultural chemical plant in Nanjing, Jiangsu province, PR China. The sludge sample
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