Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotox
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ORIGINAL PAPER
Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound Mohandas Dhaneesha . Md. Umar . Titus Susan Merlin . Kottekkatu Padinchati Krishnan . Vrinda Sukumaran . Rupesh Kumar Sinha . Abdulaziz Anas . Peng Fu . John Bryan MacMillan . Thavarool Puthiyedathu Sajeevan
Received: 12 June 2020 / Accepted: 21 October 2020 Ó Springer Nature Switzerland AG 2020
Abstract Herein we report the isolation of a novel actinomycete, strain MCCB 268T, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268T showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157T (98.06%), Pseudonocardia soli NW8-21 (97.22%) Pseudonocardia endophytica YIM 56035 (97.08%) and Pseudonocardia nantongensis KLBMP 1282 (97.34%) showing that the strain should be assigned to the genus Pseudonocardia. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01490-7) contains supplementary material, which is available to authorized users. M. Dhaneesha Md. Umar T. S. Merlin V. Sukumaran T. P. Sajeevan (&) National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala 682 016, India e-mail: [email protected] M. Dhaneesha Department of Environmental Studies, Kannur University, Mangattuparamba Campus, Kannur, Kerala 670 567, India
DNA–DNA hybridization with Pseudonocardia konjuensis LM 157T showed only 41.5% relatedness to strain MCCB 268T. The whole genome of the strain MCCB 268T was sequenced. Whole-genome average nucleotide identity, dDDH (%) and genome tree analysis demonstrated that strain significantly differed from other Pseudonocardia species. The G ? C content was 70.5 mol%. MCCB 268T exhibited in vitro cytotoxicity and through bioassay guided fractionation followed by HPLC separation a cytotoxic compound (I) was isolated. The compound (I) was identified as 1-acetyl-b-carboline through NMR spectra and high-resolution mass spectrometry. Compound (I) showed cytotoxicity against lung V. Sukumaran A. Anas CSIR-National Institute of Oceanography, Regional Centre-Cochin, Ernakulam, Kerala 682 018, India P. Fu J. B. MacMillan T. P. Sajeevan Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA J. B. MacMillan Department of Chemistry and Biochemistry, University of CA – Santa Cruz, Santa Cruz, CA 95064, USA
K. P. Krishnan R. K. Sinha National Centre for Polar and Ocean Research, Ministry of Earth Sciences, Headland Sada, Vasco da Gama, Goa 403 804, India
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Antonie van Leeuwenhoek
cancer cell line and mode of anticancer activity was found to be through the induction of apoptosis. Based on the genotypic and phenotypic features, MCCB 268T ought to be classified as a novel species under the genus Pseudonocardia for which the name Pseudonocardia cytotoxica sp. nov. is proposed (= CCUG7
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